BMRB Entry 10212

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PDB ID: 2cud
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10212
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the SH3 domain of the human SRC-like adopter protein (SLAP)

Deposition date:

2008-04-09

Original release date:

2010-03-11

Authors:

Ohnishi, S.; Kigawa, T.; Tochio, N.; Sato, M.; Nameki, N.; Koshiba, S.; Inoue, M.; Yokoyama, S.

Citation:

Citation: Ohnishi, S.; Kigawa, T.; Tochio, N.; Sato, M.; Nameki, N.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the SH3 domain of the human SRC-like adopter protein (SLAP)" .

Assembly members:

Assembly members:
SH3 domain, polymer, 79 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: Cell free synthesis Vector: P040921-09

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SH3 domain: GSSGSSGPLPNPEGLDSDFL AVLSDYPSPDISPPIFRRGE KLRVISDEGGWWKAISLSTG RESYIPGICVARVSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	304
15N chemical shifts	68
1H chemical shifts	475

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SH3 domain	1

Entities:

Entity 1, SH3 domain 79 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

PRO

LEU

PRO

2

ASN

PRO

GLU

GLY

LEU

ASP

SER

ASP

PHE

LEU

3

ALA

VAL

LEU

SER

ASP

TYR

PRO

SER

PRO

ASP

4

ILE

SER

PRO

ILE

PHE

ARG

GLY

GLU

5

LYS

LEU

ARG

VAL

ILE

SER

ASP

GLU

GLY

6

TRP

LYS

ALA

ILE

SER

LEU

SER

THR

GLY

7

ARG

GLU

SER

TYR

ILE

PRO

GLY

ILE

CYS

VAL

8

ALA

ARG

VAL

SER

GLY

PRO

SER

GLY

Samples:

sample_1: SH3 domain, [U-13C; U-15N], 1.28 mM; d-TrisHCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	not available	condition_1
3D 13C-separated NOESY	sample_1	not available	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0,4, Johnson, B. A. - data analysis

Kujira v0.9295, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 900 MHz

PDB	2CUD
DBJ	BAA13758 BAE89471 BAG35478 BAG73760 BAH12578
EMBL	CAB53536 CAG38774
GB	AAC27662 AAC50357 AAH07042 AAV38795 AAV38796
PRF	2204265A
REF	NP_001039021 NP_001039022 NP_001269893 NP_001274231 NP_006739
SP	Q13239
AlphaFold	Q13239