BMRB Entry 10194

Name: Solution structure of the SH3 domain of mouse RUN and TBC1 domain containing 3
Published: 2009-02-27

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PDB ID: 2yuo
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10194
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the SH3 domain of mouse RUN and TBC1 domain containing 3

Deposition date:

2008-02-19

Original release date:

2009-02-27

Authors:

Abe, H.; Tochio, N.; Miyamoto, K.; Saito, K.; Sasagawa, A.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Abe, H.; Tochio, N.; Miyamoto, K.; Saito, K.; Sasagawa, A.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the SH3 domain of mouse RUN and TBC1 domain containing 3" .

Assembly members:

Assembly members:
SH3 domain, polymer, 78 residues, Formula weight is not available

Natural source:

Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P060417-23

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SH3 domain: GSSGSSGRRAKALLDFERHD DDELGFRKNDIITIISQKDE HCWVGELNGLRGWFPAKFVE VLDERSKEYSIASGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	317
15N chemical shifts	72
1H chemical shifts	492

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SH3 domain	1

Entities:

Entity 1, SH3 domain 78 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ARG

ALA

2

LYS

ALA

LEU

ASP

PHE

GLU

ARG

HIS

ASP

3

ASP

GLU

LEU

GLY

PHE

ARG

LYS

ASN

ASP

4

ILE

THR

ILE

SER

GLN

LYS

ASP

GLU

5

HIS

CYS

TRP

VAL

GLY

GLU

LEU

ASN

GLY

LEU

6

ARG

GLY

TRP

PHE

PRO

ALA

LYS

PHE

VAL

GLU

7

VAL

LEU

ASP

GLU

ARG

SER

LYS

GLU

TYR

SER

8

ILE

ALA

SER

GLY

PRO

SER

GLY

Samples:

sample_1: SH3 domain, [U-13C; U-15N], 1.00 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; IDA 1 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F - processing

NMNMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9747, Kobayashi, N. - data analysis

CYANA v2.0.17, Guentert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2YUO
DBJ	BAC82539 BAE42041
EMBL	CAG04108
GB	AAH18197 AAH24122 AAH25561 AAH54597 AAH62060
REF	NP_001070013 NP_598852 NP_942082 NP_998495 XP_001097960
SP	Q2KI13 Q6P6R7 Q7T2D0 Q8VCZ6
TPG	DAA29091
AlphaFold	Q2KI13 Q6P6R7 Q7T2D0 Q8VCZ6