BMRB Entry 10165

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PDB ID: 2eef
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10165
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the CBM_21 domain from human protein phosphatase 1, regulatory (inhibitor) subunit 3B

Deposition date:

2007-12-28

Original release date:

2009-02-23

Authors:

Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the CBM_21 domain from human protein phosphatase 1, regulatory (inhibitor) subunit 3B" .

Assembly members:

Assembly members:
CBM_21 domain, residues 8-156, polymer, 156 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P060919-05

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CBM_21 domain, residues 8-156: GSSGSSGAESESFVLDFSQP SADYLDFRNRLQADHVCLEN CVLKDKAIAGTVKVQNLAFE KTVKIRMTFDTWKSYTDFPC QYVKDTYAGSDRDTFSFDIS LPEKIQSYERMEFAVYYECN GQTYWDSNRGKNYRIIRAEL KSTQGMTKPHSGPDLG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	689
15N chemical shifts	164
1H chemical shifts	1053

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Protein phosphatase 1, regulatory (Inhibitor) subunit 3B	1

Entities:

Entity 1, Protein phosphatase 1, regulatory (Inhibitor) subunit 3B 156 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ALA

GLU

SER

2

GLU

SER

PHE

VAL

LEU

ASP

PHE

SER

GLN

PRO

3

SER

ALA

ASP

TYR

LEU

ASP

PHE

ARG

ASN

ARG

4

LEU

GLN

ALA

ASP

HIS

VAL

CYS

LEU

GLU

ASN

5

CYS

VAL

LEU

LYS

ASP

LYS

ALA

ILE

ALA

GLY

6

THR

VAL

LYS

VAL

GLN

ASN

LEU

ALA

PHE

GLU

7

LYS

THR

VAL

LYS

ILE

ARG

MET

THR

PHE

ASP

8

THR

TRP

LYS

SER

TYR

THR

ASP

PHE

PRO

CYS

9

GLN

TYR

VAL

LYS

ASP

THR

TYR

ALA

GLY

SER

10

ASP

ARG

ASP

THR

PHE

SER

PHE

ASP

ILE

SER

11

LEU

PRO

GLU

LYS

ILE

GLN

SER

TYR

GLU

ARG

12

MET

GLU

PHE

ALA

VAL

TYR

GLU

CYS

ASN

13

GLY

GLN

THR

TYR

TRP

ASP

SER

ASN

ARG

GLY

14

LYS

ASN

TYR

ARG

ILE

ARG

ALA

GLU

LEU

15

LYS

SER

THR

GLN

GLY

MET

THR

LYS

PRO

HIS

16

SER

GLY

PRO

ASP

LEU

GLY

Samples:

sample_1: filamin domain, [U-13C; U-15N], 1.26 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; D2O 10%; H2O 90%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	not available	condition_1
3D 15N-separated NOESY	sample_1	not available	condition_1

Software:

TOPSPIN v1.3, Bruker - collection

NMRPipe v20060524, Delaglio, F - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9823, Kobayashi, N. - data analysis

CYANA v2.0.17, Guentert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE II 900 MHz

PDB	2EEF
DBJ	BAB14811 BAG53215
GB	AAH43388 ADQ33125 AIC57095 EAW65572 EHH63994
REF	NP_001188258 NP_078883 XP_003902491 XP_003902492 XP_004046665
SP	Q86XI6
AlphaFold	Q86XI6