BMRB Entry 10149

Name: Solution structure of the CH domain from human MICAL-2
Published: 2008-12-11

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PDB ID: 2e9k
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10149
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the CH domain from human MICAL-2

Deposition date:

2007-12-11

Original release date:

2008-12-11

Authors:

Tomizawa, T.; Tochio, N.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Tomizawa, T.; Tochio, N.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the CH domain from human MICAL-2" .

Assembly members:

Assembly members:
CH domain, polymer, 121 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P060522-24

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CH domain: GSSGSSGDIRPSKLLTWCQQ QTEGYQHVNVTDLTTSWRSG LALCAIIHRFRPELINFDSL NEDDAVENNQLAFDVAEREF GIPPVTTGKEMASAQEPDKL SMVMYLSKFYELFRGTPLRP V

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	529
15N chemical shifts	123
1H chemical shifts	835

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Protein MICAL-2	1

Entities:

Entity 1, Protein MICAL-2 121 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ASP

ILE

ARG

2

PRO

SER

LYS

LEU

THR

TRP

CYS

GLN

3

GLN

THR

GLU

GLY

TYR

GLN

HIS

VAL

ASN

VAL

4

THR

ASP

LEU

THR

SER

TRP

ARG

SER

GLY

5

LEU

ALA

LEU

CYS

ALA

ILE

HIS

ARG

PHE

6

ARG

PRO

GLU

LEU

ILE

ASN

PHE

ASP

SER

LEU

7

ASN

GLU

ASP

ALA

VAL

GLU

ASN

GLN

8

LEU

ALA

PHE

ASP

VAL

ALA

GLU

ARG

GLU

PHE

9

GLY

ILE

PRO

VAL

THR

GLY

LYS

GLU

10

MET

ALA

SER

ALA

GLN

GLU

PRO

ASP

LYS

LEU

11

SER

MET

VAL

MET

TYR

LEU

SER

LYS

PHE

TYR

12

GLU

LEU

PHE

ARG

GLY

THR

PRO

LEU

ARG

PRO

13

VAL

Samples:

sample_1: CH domain, [U-13C; U-15N], 1.20 mM; d-Tris-HCl(pH 7.0) 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	not available	condition_1
3D 15N-separated NOESY	sample_1	not available	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20060524, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9747, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 900 MHz

PDB	2E9K
GB	EFB30164
REF	XP_003919889 XP_004394651 XP_004394652 XP_004394653 XP_004394654