BMRB Entry 10148

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PDB ID: 2ee8
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10148
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of three zf-C2H2 domains from mouse protein odd-skipped-related 2 splicing isoform 2

Deposition date:

2007-12-04

Original release date:

2008-12-10

Authors:

Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of three zf-C2H2 domains from mouse protein odd-skipped-related 2 splicing isoform 2" .

Assembly members:

Assembly members:
zf-C2H2 domains, polymer, 106 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P060529-15

Entity Sequences (FASTA):

Entity Sequences (FASTA):
zf-C2H2 domains: GSSGSSGGRLPSKTKKEFIC KFCGRHFTKSYNLLIHERTH TDERPYTCDICHKAFRRQDH LRDHRYIHSKEKPFKCQECG KGFCQSRTLAVHKTLHMQTS SPTAAS

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	456
15N chemical shifts	89
1H chemical shifts	679

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	zf-C2H2 domains	1
2	ZINC ION No.1	2
3	ZINC ION No.2	2
4	ZINC ION No.3	2

Entities:

Entity 1, zf-C2H2 domains 106 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ARG

LEU

2

PRO

SER

LYS

THR

LYS

GLU

PHE

ILE

CYS

3

LYS

PHE

CYS

GLY

ARG

HIS

PHE

THR

LYS

SER

4

TYR

ASN

LEU

ILE

HIS

GLU

ARG

THR

HIS

5

THR

ASP

GLU

ARG

PRO

TYR

THR

CYS

ASP

ILE

6

CYS

HIS

LYS

ALA

PHE

ARG

GLN

ASP

HIS

7

LEU

ARG

ASP

HIS

ARG

TYR

ILE

HIS

SER

LYS

8

GLU

LYS

PRO

PHE

LYS

CYS

GLN

GLU

CYS

GLY

9

LYS

GLY

PHE

CYS

GLN

SER

ARG

THR

LEU

ALA

10

VAL

HIS

LYS

THR

LEU

HIS

MET

GLN

THR

SER

11

SER

PRO

THR

ALA

SER

Entity 2, ZINC ION No.1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: zf-C2H2 domains, [U-13C; U-15N], 0.86 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	not available	condition_1
3D 15N-separated NOESY	sample_1	not available	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20060524, Delaglio, F - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9823, Kobayashi, N. - data analysis

CYANA v2.0.17, Guentert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 700 MHz

PDB	2EE8
DBJ	BAC34859 BAF84180
GB	AAH13504 AAH16936 AAH79211 AAI02144 AAI08580
REF	NP_001012118 NP_001029500 NP_001089872 NP_001090353 NP_001163815
SP	Q0IHB8 Q32NK7 Q3T135 Q6AY34
TPG	DAA22584
AlphaFold	Q0IHB8 Q32NK7 Q3T135 Q6AY34