BMRB Entry 10146

Name: Solution structure of the 21th filamin domain from human Filamin-B
Published: 2008-12-10

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PDB ID: 2ee6
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10146
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the 21th filamin domain from human Filamin-B

Deposition date:

2007-12-04

Original release date:

2008-12-10

Authors:

Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the 21th filamin domain from human Filamin-B" .

Assembly members:

Assembly members:
filamin domain, polymer, 105 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P060508-07

Entity Sequences (FASTA):

Entity Sequences (FASTA):
filamin domain: GSSGSSGEGGAHKVRAGGPG LERGEAGVPAEFSIWTREAG AGGLSIAVEGPSKAEITFDD HKNGSCGVSYIAQEPGNYEV SIKFNDEHIPESPYLVPVIA PSDDA

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	397
15N chemical shifts	92
1H chemical shifts	629

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Filamin-B	1

Entities:

Entity 1, Filamin-B 105 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

GLU

GLY

2

ALA

HIS

LYS

VAL

ARG

ALA

GLY

PRO

GLY

3

LEU

GLU

ARG

GLY

GLU

ALA

GLY

VAL

PRO

ALA

4

GLU

PHE

SER

ILE

TRP

THR

ARG

GLU

ALA

GLY

5

ALA

GLY

LEU

SER

ILE

ALA

VAL

GLU

GLY

6

PRO

SER

LYS

ALA

GLU

ILE

THR

PHE

ASP

7

HIS

LYS

ASN

GLY

SER

CYS

GLY

VAL

SER

TYR

8

ILE

ALA

GLN

GLU

PRO

GLY

ASN

TYR

GLU

VAL

9

SER

ILE

LYS

PHE

ASN

ASP

GLU

HIS

ILE

PRO

10

GLU

SER

PRO

TYR

LEU

VAL

PRO

VAL

ILE

ALA

11

PRO

SER

ASP

ALA

Samples:

sample_1: filamin domain, [U-13C; U-15N], 1.15 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	not available	condition_1
3D 15N-separated NOESY	sample_1	not available	condition_1

Software:

TOPSPIN v1.3, Bruker - collection

NMRPipe v20060524, Delaglio, F - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9823, Kobayashi, N. - data analysis

CYANA v2.0.17, Guentert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE II 900 MHz

PDB	2EE6
REF	XP_006751735