BMRB Entry 10143

Name: Solution structure of the 14th filamin domain from human Filamin-B
Published: 2008-12-10

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PDB ID: 2e9j
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10143
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the 14th filamin domain from human Filamin-B

Deposition date:

2007-12-04

Original release date:

2008-12-10

Authors:

Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the 14th filamin domain from human Filamin-B" .

Assembly members:

Assembly members:
filamin domain, polymer, 119 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P060619-05

Entity Sequences (FASTA):

Entity Sequences (FASTA):
filamin domain: GSSGSSGRSPFKVKVLPTYD ASKVTASGPGLSSYGVPASL PVDFAIDARDAGEGLLAVQI TDQEGKPKRAIVHDNKDGTY AVTYIPDKTGRYMIGVTYGG DDIPLSPYRIRATQTGDAS

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	477
15N chemical shifts	106
1H chemical shifts	741

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Filamin-B	1

Entities:

Entity 1, Filamin-B 119 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ARG

SER

PRO

2

PHE

LYS

VAL

LYS

VAL

LEU

PRO

THR

TYR

ASP

3

ALA

SER

LYS

VAL

THR

ALA

SER

GLY

PRO

GLY

4

LEU

SER

TYR

GLY

VAL

PRO

ALA

SER

LEU

5

PRO

VAL

ASP

PHE

ALA

ILE

ASP

ALA

ARG

ASP

6

ALA

GLY

GLU

GLY

LEU

ALA

VAL

GLN

ILE

7

THR

ASP

GLN

GLU

GLY

LYS

PRO

LYS

ARG

ALA

8

ILE

VAL

HIS

ASP

ASN

LYS

ASP

GLY

THR

TYR

9

ALA

VAL

THR

TYR

ILE

PRO

ASP

LYS

THR

GLY

10

ARG

TYR

MET

ILE

GLY

VAL

THR

TYR

GLY

11

ASP

ILE

PRO

LEU

SER

PRO

TYR

ARG

ILE

12

ARG

ALA

THR

GLN

THR

GLY

ASP

ALA

SER

Samples:

sample_1: filamin domain, [U-13C; U-15N], 1.19 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	not available	condition_1
3D 15N-separated NOESY	sample_1	not available	condition_1

Software:

TOPSPIN v1.3, Bruker - collection

NMRPipe v20060524, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9747, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE II 900 MHz