BMRB Entry 10136

Name: Solution structure of the first SH3 domain of human intersectin2 (KIAA1256)
Published: 2008-09-26

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PDB ID: 1uff
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10136
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the first SH3 domain of human intersectin2 (KIAA1256)

Deposition date:

2007-05-14

Original release date:

2008-09-26

Authors:

Kobayashi, N.; Kigawa, T.; Koshiba, S.; Tochio, N.; Inoue, M.; Yokoyama, S.

Citation:

Citation: Kobayashi, N.; Kigawa, T.; Koshiba, S.; Tochio, N.; Inoue, M.; Yokoyama, S.. "Solution structure of the first SH3 domain of human intersectin2 (KIAA1256)" .

Assembly members:

Assembly members:
Intersectin 2, polymer, 93 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P021015-27

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Intersectin 2: GSSGSSGYRALYPFEARNHD EMSFNSGDIIQVDEKTVGEP GWLYGSFQGNFGWFPCNYVE KMPSSENEKAVSPKKALLPP TVSLSATSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	392
15N chemical shifts	92
1H chemical shifts	576

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Intersectin 2	1

Entities:

Entity 1, Intersectin 2 93 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

TYR

ARG

ALA

2

LEU

TYR

PRO

PHE

GLU

ALA

ARG

ASN

HIS

ASP

3

GLU

MET

SER

PHE

ASN

SER

GLY

ASP

ILE

4

GLN

VAL

ASP

GLU

LYS

THR

VAL

GLY

GLU

PRO

5

GLY

TRP

LEU

TYR

GLY

SER

PHE

GLN

GLY

ASN

6

PHE

GLY

TRP

PHE

PRO

CYS

ASN

TYR

VAL

GLU

7

LYS

MET

PRO

SER

GLU

ASN

GLU

LYS

ALA

8

VAL

SER

PRO

LYS

ALA

LEU

PRO

9

THR

VAL

SER

LEU

SER

ALA

THR

SER

GLY

PRO

10

SER

GLY

Samples:

sample_1: SH3 domain, [U-13C; U-15N], 0.54 mM; phosphate 20 mM; NaCl 100 mM; d10-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.816, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz