BMRB Entry 10132

Name: 1H and 15N assignment of oxidized Pseudomonas aeruginosa cytochrome c551
Published: 2008-02-11

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PDB ID: 2pac
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR10132
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H and 15N assignment of oxidized Pseudomonas aeruginosa cytochrome c551

Deposition date:

2007-04-15

Original release date:

2008-02-11

Authors:

Takayama, Shin-ichi; Takahashi, Yo-ta; Mikami, Shin-ichi; Irie, Kiyofumi; Kawano, Shin; Yamamoto, Yasuhiko; Hemmi, Hikaru; Kitahara, Ryo; Yokoyama, Shigeyuki; Akasaka, Kazuyuki

Citation:

Citation: Takayama, Shin-ichi; Takahashi, Yo-ta; Mikami, Shin-ichi; Irie, Kiyofumi; Kawano, Shin; Yamamoto, Yasuhiko; Hemmi, Hikaru; Kitahara, Ryo; Yokoyama, Shigeyuki; Akasaka, Kazuyuki. "Local conformational transition of Hydrogenobacter thermophilus cytochrome c552 relevant to its redox potential." Biochemistry 46, 9215-9224 (2007).
PubMed: 17658890

Assembly members:

Assembly members:
cytochrome c551, polymer, 82 residues, Formula weight is not available
HEC, non-polymer, 618.503 Da.

Natural source:

Natural source: Common Name: Pseudomonas aeruginosa Taxonomy ID: 287 Superkingdom: Eubacteria Kingdom: not available Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source: Production method: recombinant technology Vector: pKK223-3

Entity Sequences (FASTA):

Entity Sequences (FASTA):
cytochrome c551: EDPEVLFKNKGCVACHAIDT KMVGPAYKDVAAKFAGQAGA EAELAQRIKNGSQGVWGPIP MPPNAVSDDEAQTLAKWVLS QK

Data sets:

assigned_chemical_shifts
- shift_1

Data type	Count
15N chemical shifts	74
1H chemical shifts	74

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	cytochrome c	1
2	heme	2

Entities:

Entity 1, cytochrome c 82 residues - Formula weight is not available

1

GLU

ASP

PRO

GLU

VAL

LEU

PHE

LYS

ASN

LYS

2

GLY

CYS

VAL

ALA

CYS

HIS

ALA

ILE

ASP

THR

3

LYS

MET

VAL

GLY

PRO

ALA

TYR

LYS

ASP

VAL

4

ALA

LYS

PHE

ALA

GLY

GLN

ALA

GLY

ALA

5

GLU

ALA

GLU

LEU

ALA

GLN

ARG

ILE

LYS

ASN

6

GLY

SER

GLN

GLY

VAL

TRP

GLY

PRO

ILE

PRO

7

MET

PRO

ASN

ALA

VAL

SER

ASP

GLU

8

ALA

GLN

THR

LEU

ALA

LYS

TRP

VAL

LEU

SER

9

GLN

LYS

Entity 2, heme - C34 H34 Fe N4 O4 - 618.503 Da.

1

HEC

Samples:

sample_1: cytochrome c551, [U-15N], 1 mM; potassium phosphate 20 mM; potassium ferricyanide 10 mM

Cond_1: pH: 6.0; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H15N HSQC	sample_1	not available	Cond_1

Software:

No software information available

NMR spectrometers:

Bruker Avance 600 MHz

BMRB	10133 1333 1334 1734 1866 2968
PDB	2EXV 2PAC 351C 3X39 451C
DBJ	BAD11780 BAK92489 BAP19663 BAP48532 BAQ37290
EMBL	CAA35703 CAA35958 CAW25242 CCQ85614 CDH68848
GB	AAG03907 AAT49429 ABJ15480 ABR83995 AEO73006
PRF	630481A
REF	NP_249209 WP_003084872 WP_003118863 WP_003129143 WP_012074083
SP	P00099
AlphaFold	P00099