BMRB Entry 10129

Name: Solution structure of the first Fn3 domain of Sidekick-2 protein
Published: 2008-09-02

Click here to enlarge.

PDB ID: 1wf5
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10129
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Solution structure of the first Fn3 domain of Sidekick-2 protein

Deposition date:

2007-04-02

Original release date:

2008-09-02

Authors:

Qin, X.; Hayashi, F.; Yokoyama, S.

Citation:

Citation: Qin, X.; Hayashi, F.; Yokoyama, S.. "Solution structure of the first Fn3 domain of Sidekick-2 protein" .

Assembly members:

Assembly members:
FNIII domain, polymer, 121 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P040223-40

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FNIII domain: GSSGSSGRSAHLRVRQLPHA PEHPVATLSTVERRAINLTW TKPFDGNSPLIRYILEMSEN NAPWTVLLASVDPKATSVTV KGLVPARSYQFRLCAVNDVG KGQFSKDTERVSLPESGPSS G

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	514
15N chemical shifts	115
1H chemical shifts	809

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	sidekick 2 protein	1

Entities:

Entity 1, sidekick 2 protein 121 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ARG

SER

ALA

2

HIS

LEU

ARG

VAL

ARG

GLN

LEU

PRO

HIS

ALA

3

PRO

GLU

HIS

PRO

VAL

ALA

THR

LEU

SER

THR

4

VAL

GLU

ARG

ALA

ILE

ASN

LEU

THR

TRP

5

THR

LYS

PRO

PHE

ASP

GLY

ASN

SER

PRO

LEU

6

ILE

ARG

TYR

ILE

LEU

GLU

MET

SER

GLU

ASN

7

ASN

ALA

PRO

TRP

THR

VAL

LEU

ALA

SER

8

VAL

ASP

PRO

LYS

ALA

THR

SER

VAL

THR

VAL

9

LYS

GLY

LEU

VAL

PRO

ALA

ARG

SER

TYR

GLN

10

PHE

ARG

LEU

CYS

ALA

VAL

ASN

ASP

VAL

GLY

11

LYS

GLY

GLN

PHE

SER

LYS

ASP

THR

GLU

ARG

12

VAL

SER

LEU

PRO

GLU

SER

GLY

PRO

SER

13

GLY

Samples:

sample_1: FNIII domain, [U-13C; U-15N], 1.29 mM; PiNa 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.854, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

Varian INOVA 800 MHz