BMRB Entry 10125

Name: Solution structure of the dsRBD from hypothetical protein BAB26260
Published: 2008-09-02

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PDB ID: 1whn
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10125
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the dsRBD from hypothetical protein BAB26260

Deposition date:

2007-04-02

Original release date:

2008-09-02

Authors:

Nagata, T.; Muto, Y.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.

Citation:

Citation: Nagata, T.; Muto, Y.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.. "Solution structure of the dsRBD from hypothetical protein BAB26260" .

Assembly members:

Assembly members:
double-stranded RNA binding domain, polymer, 128 residues, Formula weight is not available

Natural source:

Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P021209-40

Entity Sequences (FASTA):

Entity Sequences (FASTA):
double-stranded RNA binding domain: GSSGSSGSGIIKMAIRFDRR AYPPQITPKMCLLEWCRREK LPQPVYETVQRTIDRMFCSV VTVAEQKYQSTLWDKSKKLA EQTAAIVCLRSQGLPEGRLG EESPSLNKRKREAPDQDPGG PRSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	547
15N chemical shifts	124
1H chemical shifts	857

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	hypothetical protein RIKEN cDNA 2310016K04	1

Entities:

Entity 1, hypothetical protein RIKEN cDNA 2310016K04 128 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

SER

GLY

ILE

2

ILE

LYS

MET

ALA

ILE

ARG

PHE

ASP

ARG

3

ALA

TYR

PRO

GLN

ILE

THR

PRO

LYS

MET

4

CYS

LEU

GLU

TRP

CYS

ARG

GLU

LYS

5

LEU

PRO

GLN

PRO

VAL

TYR

GLU

THR

VAL

GLN

6

ARG

THR

ILE

ASP

ARG

MET

PHE

CYS

SER

VAL

7

VAL

THR

VAL

ALA

GLU

GLN

LYS

TYR

GLN

SER

8

THR

LEU

TRP

ASP

LYS

SER

LYS

LEU

ALA

9

GLU

GLN

THR

ALA

ILE

VAL

CYS

LEU

ARG

10

SER

GLN

GLY

LEU

PRO

GLU

GLY

ARG

LEU

GLY

11

GLU

SER

PRO

SER

LEU

ASN

LYS

ARG

LYS

12

ARG

GLU

ALA

PRO

ASP

GLN

ASP

PRO

GLY

13

PRO

ARG

SER

GLY

PRO

SER

GLY

Samples:

sample_1: double-stranded RNA binding domain, [U-13C; U-15N], 1.28 mM; phosphate 20 mM; NaCl 100 mM; DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 100 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

NMRView v5.0.4, Johnson - data analysis

CYANA v2.0, Guntert, P. - refinement, structure solution

NMRPipe v2.3, Delaglio, F. - processing

Kujira v0.901, Kobayashi, N. - data analysis

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	1WHN
DBJ	BAB26260
GB	AAH58431 AAI16935 AAI16937 EDL11340 EDL11341
REF	NP_001288105 NP_079794 XP_006531341 XP_006986898
SP	Q9D7B1
AlphaFold	Q9D7B1