BMRB Entry 10123

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PDB ID: 1n27
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10123
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the PWWP domain of mouse Hepatoma-derived growth factor, related protein 3

Deposition date:

2007-04-02

Original release date:

2008-07-16

Authors:

Nameki, N.; Kigawa, T.; Koshiba, S.; Kobayashi, N.; Tochio, N.; Inoue, M.; Yokoyama, S.

Citation:

Citation: Nameki, N.; Tochio, N.; Koshiba, S.; Inoue, M.; Yabuki, T.; Aoki, M.; Seki, E.; Matsuda, T.; Fujikura, Y.; Saito, M.; Ikari, M.; Watanabe, M.; Terada, T.; Shirouzu, M.; Yoshida, M.; Hirota, H.; Tanaka, A.; Hayashizaki, Y.; Guntert, P.; Kigawa, T.; Yokoyama, S.. "Solution structure of the PWWP domain of the hepatoma-derived growth factor family." Protein Sci. 14, 756-764 (2005).
PubMed: 15689505

Assembly members:

Assembly members:
PWWP domain, polymer, 96 residues, Formula weight is not available

Natural source:

Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P020408-01

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PWWP domain: GSSGSSGEYKAGDLVFAKMK GYPHWPARIDELPEGAVKPP ANKYPIFFFGTHETAFLGPK DLFPYKEYKDKFGKSNKRKG FNEGLWEIENSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	438
15N chemical shifts	91
1H chemical shifts	644

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PWWP domain	1

Entities:

Entity 1, PWWP domain 96 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

GLU

TYR

LYS

2

ALA

GLY

ASP

LEU

VAL

PHE

ALA

LYS

MET

LYS

3

GLY

TYR

PRO

HIS

TRP

PRO

ALA

ARG

ILE

ASP

4

GLU

LEU

PRO

GLU

GLY

ALA

VAL

LYS

PRO

5

ALA

ASN

LYS

TYR

PRO

ILE

PHE

GLY

6

THR

HIS

GLU

THR

ALA

PHE

LEU

GLY

PRO

LYS

7

ASP

LEU

PHE

PRO

TYR

LYS

GLU

TYR

LYS

ASP

8

LYS

PHE

GLY

LYS

SER

ASN

LYS

ARG

LYS

GLY

9

PHE

ASN

GLU

GLY

LEU

TRP

GLU

ILE

GLU

ASN

10

SER

GLY

PRO

SER

GLY

Samples:

sample_1: PWWP domain, [U-13C; U-15N], 0.7 mM; phosphate 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.613, Kobayashi, N. - data analysis

CYANA v1.0.6, Guntert, P. - structure solution

OPALp, Billeter, M., Guntert, P., Koradi, R. - refinement

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	1N27
DBJ	BAA90477 BAA90478 BAA91597 BAB31754 BAC32123
GB	AAD34137 AAH15483 AAH55734 AAI51803 AAK72965
REF	NP_001178297 NP_001297902 NP_038914 NP_057157 NP_665728
SP	Q923W4 Q9JMG7 Q9Y3E1
TPG	DAA17616
AlphaFold	Q923W4 Q9JMG7 Q9Y3E1