BMRB Entry 10118

Name: 1H 13C and 15N Chemical Shift Assignments for C-terminal domain of PH0471
Published: 2008-09-22

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PDB ID: 2exd
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10118
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H 13C and 15N Chemical Shift Assignments for C-terminal domain of PH0471

Deposition date:

2007-03-07

Original release date:

2008-09-22

Authors:

Kuwahara, Yohta; Ohno, Ayako; Morii, Taichi; Tochio, Hidehito; Shirakawa, Masahiro; Hiroaki, Hidekazu

Citation:

Citation: Kuwahara, Yohta; Ohno, Ayako; Morii, Taichi; Yokoyama, Hideshi; Matsui, Ikuo; Tochio, Hidehito; Shirakawa, Masahiro; Hiroaki, Hidekazu. "The solution structure of the C-terminal domain of NfeD reveals a novel membrane-anchored OB-fold" Protein Sci. 17, 1915-1924 (2008).
PubMed: 18687870

Assembly members:

Assembly members:
PH0471 C-terminal domain, polymer, 74 residues, 8360 Da.

Natural source:

Natural source: Common Name: Pyrococcus horikoshii OT3 Taxonomy ID: 70601 Superkingdom: Archaea Kingdom: not available Genus/species: Pyrococcus horikoshii

Experimental source:

Experimental source: Production method: recombinant technology Vector: pET21b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PH0471 C-terminal domain: RRETTDIGGGKYTFELKGKV GKVVKIAEDHYLVEVEGDKW IAYSDEKLSLGDRVMVVDVD GLKLKVKRIPPQLE

Data sets:

assigned_chemical_shifts
- chemical_shift1

Data type	Count
13C chemical shifts	270
15N chemical shifts	74
1H chemical shifts	552

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PH0471_C-terminal_domain	1

Entities:

Entity 1, PH0471_C-terminal_domain 74 residues - 8360 Da.

1

ARG

GLU

THR

ASP

ILE

GLY

2

LYS

TYR

THR

PHE

GLU

LEU

LYS

GLY

LYS

VAL

3

GLY

LYS

VAL

LYS

ILE

ALA

GLU

ASP

HIS

4

TYR

LEU

VAL

GLU

VAL

GLU

GLY

ASP

LYS

TRP

5

ILE

ALA

TYR

SER

ASP

GLU

LYS

LEU

SER

LEU

6

GLY

ASP

ARG

VAL

MET

VAL

ASP

VAL

ASP

7

GLY

LEU

LYS

LEU

LYS

VAL

LYS

ARG

ILE

PRO

8

PRO

GLN

LEU

GLU

Samples:

sample_1: PH0471 C-terminal domain, [U-13C; U-15N], 0.7 mM; Na Phosphate 25 mM; NaCl 300 mM; EDTA 1 mM; H2O 95%; D2O 5%

sample_2: PH0471 C-terminal domain, [U-15N], 1 mM; Na Phosphate 25 mM; NaCl 300 mM; EDTA 1 mM; H2O 95%; D2O 5%

condition_1: ionic strength: 300 mM; pH: 5.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HSQC	sample_2	not available	condition_1
1H-13C HSQC	sample_1	not available	condition_1
HNCA	sample_1	not available	condition_1
HNCACB	sample_1	not available	condition_1
CBCACONH	sample_1	not available	condition_1
HCC(CO)NH	sample_1	not available	condition_1
CC(CO)NH	sample_1	not available	condition_1
HCCH-TOCSY	sample_1	not available	condition_1

Software:

XWIN-NMR v3.5, Bruker - collection

NMRPipe v2.3 - collection, FT

SPARKY v3.106 - assignment

CYANA v2.0.17, PETER GUNTERT - refinement, structure solution

VNMR v6.1C, VARIAN - collection

NMR spectrometers:

Bruker DMX 800 MHz
Bruker DMX 500 MHz
Varian INOVA 900 MHz

PDB	2EXD
DBJ	BAA29559
REF	WP_010884582