BMRB Entry 10115

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10115
MolProbity Validation Chart

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Title:
Solution Structure of the Pleckstrin Homology Domain of Oxysterol-Binding Protein-Related Protein 8 (KIAA1451 Protein)
Deposition date:
2007-02-15
Original release date:
2008-08-15
Authors:
Li, H.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Li, H.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the Pleckstrin Homology Domain of Oxysterol-Binding Protein-Related Protein 8 (KIAA1451 Protein)"  .

Assembly members:

Assembly members:
Pleckstrin Homology (PH) Domain, polymer, 130 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P030428-85

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Pleckstrin Homology (PH) Domain: GSSGSSGIVMADWLKIRGTL KSWTKLWCVLKPGVLLIYKT QKNGQWVGTVLLNACEIIER PSKKDGFCFKLFHPLEQSIW AVKGPKGEAVGSITQPLPSS YLIIRATSESDGRCWMDALE LALKSGPSSG

Data sets:
Data typeCount
13C chemical shifts573
15N chemical shifts132
1H chemical shifts915

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Oxysterol binding protein-related protein 81

Entities:

Entity 1, Oxysterol binding protein-related protein 8 130 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYILEVALMET
2   ALAASPTRPLEULYSILEARGGLYTHRLEU
3   LYSSERTRPTHRLYSLEUTRPCYSVALLEU
4   LYSPROGLYVALLEULEUILETYRLYSTHR
5   GLNLYSASNGLYGLNTRPVALGLYTHRVAL
6   LEULEUASNALACYSGLUILEILEGLUARG
7   PROSERLYSLYSASPGLYPHECYSPHELYS
8   LEUPHEHISPROLEUGLUGLNSERILETRP
9   ALAVALLYSGLYPROLYSGLYGLUALAVAL
10   GLYSERILETHRGLNPROLEUPROSERSER
11   TYRLEUILEILEARGALATHRSERGLUSER
12   ASPGLYARGCYSTRPMETASPALALEUGLU
13   LEUALALEULYSSERGLYPROSERSERGLY

Samples:

sample_1: Pleckstrin Homology domain, [U-13C; U-15N], 1.21 mM; Phosphate 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1not availablecondition_1
3D 13C-separated NOESYsample_1not availablecondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.854, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAA95975 BAB71026 BAF82686 BAG11327
EMBL CAH18345 CAH91148 CAI46050
GB AAG53411 AAH82235 AAH93834 AAI01530 AAI11729
REF NP_001003712 NP_001003717 NP_001120370 NP_001125671 NP_001247808
SP B9EJ86 Q9BZF1
TPG DAA30137
AlphaFold B9EJ86 Q9BZF1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks