BMRB Entry 10110

Name: Solution Structure of the Gas2 Domain of the Growth Arrest Specific 2 Protein
Published: 2008-08-29

Click here to enlarge.

PDB ID: 1v5r
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10110
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Solution Structure of the Gas2 Domain of the Growth Arrest Specific 2 Protein

Deposition date:

2007-02-13

Original release date:

2008-08-29

Authors:

Miyamoto, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Miyamoto, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the Gas2 Domain of the Growth Arrest Specific 2 Protein" .

Assembly members:

Assembly members:
Growth-arrest-specific protein 2, polymer, 97 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P020417-07

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Growth-arrest-specific protein 2: GSSGSSGNLLDDAVKRISED PPCKCPTKFCVERLSQGRYR VGEKILFIRMLHNKHVMVRV GGGWETFAGYLLKHDPCRML QISRVDGKTSPSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	378
15N chemical shifts	79
1H chemical shifts	619

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Growth-arrest-specific protein 2	1
2	zinc ion	2

Entities:

Entity 1, Growth-arrest-specific protein 2 97 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ASN

LEU

2

ASP

ALA

VAL

LYS

ARG

ILE

SER

GLU

ASP

3

PRO

CYS

LYS

CYS

PRO

THR

LYS

PHE

CYS

4

VAL

GLU

ARG

LEU

SER

GLN

GLY

ARG

TYR

ARG

5

VAL

GLY

GLU

LYS

ILE

LEU

PHE

ILE

ARG

MET

6

LEU

HIS

ASN

LYS

HIS

VAL

MET

VAL

ARG

VAL

7

GLY

TRP

GLU

THR

PHE

ALA

GLY

TYR

8

LEU

LYS

HIS

ASP

PRO

CYS

ARG

MET

LEU

9

GLN

ILE

SER

ARG

VAL

ASP

GLY

LYS

THR

SER

10

PRO

SER

GLY

PRO

SER

GLY

Entity 2, zinc ion - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: Gas2 Domain, [U-13C; U-15N], 1.03 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 100 uM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.863, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	1V5R
DBJ	BAB29405 BAG36475 BAJ20935
EMBL	CAG29281
GB	AAA37660 AAC52058 AAH13456 AAH40470 AAH53446
REF	NP_001098471 NP_001120976 NP_001137302 NP_001244553 NP_005247
SP	O43903 P11862
TPG	DAA14022
AlphaFold	O43903 P11862