BMRB Entry 10107

Name: Solution structure of the CH domain from mouse EB-1
Published: 2008-08-15

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PDB ID: 1v5k
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10107
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the CH domain from mouse EB-1

Deposition date:

2007-02-13

Original release date:

2008-08-15

Authors:

Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.

Citation:

Citation: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the CH domain from mouse EB-1" .

Assembly members:

Assembly members:
CH domain, polymer, 115 residues, Formula weight is not available

Natural source:

Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P021216-20

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CH domain: GSSGSSGQRRHDMLAWINES LQLNLTKIEQLCSGAAYCQF MDMLFPGSIALKKVKFQAKL EHEYIQNFKILQAGFKRMGV DKIIPVDKLVKGKFQDNFEF VQWFKKFFDSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	539
15N chemical shifts	125
1H chemical shifts	834

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	microtubule-associated protein, RP/EB family, member 1	1

Entities:

Entity 1, microtubule-associated protein, RP/EB family, member 1 115 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

GLN

ARG

2

HIS

ASP

MET

LEU

ALA

TRP

ILE

ASN

GLU

SER

3

LEU

GLN

LEU

ASN

LEU

THR

LYS

ILE

GLU

GLN

4

LEU

CYS

SER

GLY

ALA

TYR

CYS

GLN

PHE

5

MET

ASP

MET

LEU

PHE

PRO

GLY

SER

ILE

ALA

6

LEU

LYS

VAL

LYS

PHE

GLN

ALA

LYS

LEU

7

GLU

HIS

GLU

TYR

ILE

GLN

ASN

PHE

LYS

ILE

8

LEU

GLN

ALA

GLY

PHE

LYS

ARG

MET

GLY

VAL

9

ASP

LYS

ILE

PRO

VAL

ASP

LYS

LEU

VAL

10

LYS

GLY

LYS

PHE

GLN

ASP

ASN

PHE

GLU

PHE

11

VAL

GLN

TRP

PHE

LYS

PHE

ASP

SER

12

GLY

PRO

SER

GLY

Samples:

sample_1: CH domain, [U-13C; U-15N], 1.03 mM; sodium phosphate 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	not available	condition_1
3D 15N-separated NOESY	sample_1	not available	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.863, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	1V5K
DBJ	BAG59745
REF	XP_003273569 XP_004062039 XP_004325232 XP_004612629 XP_004697982