BMRB Entry 10106
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10106
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Yoneyama, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the SWIB/MDM2 domain of the hypothetical protein At5g08430
from Arabidopsis thaliana" .
Assembly members:
SWIB/MDM2 domain, polymer, 101 residues, Formula weight is not available
Natural source: Common Name: thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana
Experimental source: Production method: cell free synthesis Vector: P030128-16
Entity Sequences (FASTA):
SWIB/MDM2 domain: GSSGSSGKRFEFVGWGSRQL
IEFLHSLGKDTSEMISRYDV
SDTIAKYISKEGLLDPSNKK
KVVCDKRLVLLFGTRTIFRM
KVYDLLEKHYKENQDSGPSS
G
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 446 |
| 15N chemical shifts | 99 |
| 1H chemical shifts | 695 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | hypothetical protein RAFL09-47-K03 | 1 |
Entities:
Entity 1, hypothetical protein RAFL09-47-K03 101 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | LYS | ARG | PHE | ||||
| 2 | GLU | PHE | VAL | GLY | TRP | GLY | SER | ARG | GLN | LEU | ||||
| 3 | ILE | GLU | PHE | LEU | HIS | SER | LEU | GLY | LYS | ASP | ||||
| 4 | THR | SER | GLU | MET | ILE | SER | ARG | TYR | ASP | VAL | ||||
| 5 | SER | ASP | THR | ILE | ALA | LYS | TYR | ILE | SER | LYS | ||||
| 6 | GLU | GLY | LEU | LEU | ASP | PRO | SER | ASN | LYS | LYS | ||||
| 7 | LYS | VAL | VAL | CYS | ASP | LYS | ARG | LEU | VAL | LEU | ||||
| 8 | LEU | PHE | GLY | THR | ARG | THR | ILE | PHE | ARG | MET | ||||
| 9 | LYS | VAL | TYR | ASP | LEU | LEU | GLU | LYS | HIS | TYR | ||||
| 10 | LYS | GLU | ASN | GLN | ASP | SER | GLY | PRO | SER | SER | ||||
| 11 | GLY |
Samples:
sample_1: SWIB/MDM2 domain, [U-13C; U-15N], 0.4 mM; phosphate 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | not available | condition_1 |
| 3D 13C-separated NOESY | sample_1 | not available | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20020425, Delaglio, F. - processing
NMRView v5.0.4, Jhonson, B.A. - data analysis
Kujira v0.815, Kobayashi, N. - data analysis
CYANA v1.0.7, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| EMBL | CAC08343 |
| GB | ACF06124 AED91300 |
| REF | NP_196460 |
| SP | Q9FT92 |
| AlphaFold | Q9FT92 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks