BMRB Entry 10097

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10097
MolProbity Validation Chart

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Title:
Solution Structure of the SH3 Domain Binding Glutamic Acid-rich Protein Like 3
Deposition date:
2007-02-13
Original release date:
2008-08-15
Authors:
Miyamoto, K.; Kigawa, T.; Koshiba, S.; Kobayashi, N.; Tochio, N.; Inoue, M.; Yokoyama, S.
Citation:

Citation: Miyamoto, K.; Kigawa, T.; Koshiba, S.; Kobayashi, N.; Tochio, N.; Inoue, M.; Yokoyama, S.. "Solution Structure of the SH3 Domain Binding Glutamic Acid-rich Protein Like 3"  .

Assembly members:

Assembly members:
SH3 domain-binding glutamic acid-rich-like protein 3 (SH3BGR), polymer, 100 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P020401-39

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SH3 domain-binding glutamic acid-rich-like protein 3 (SH3BGR): GSEGAATMSGLRVYSTSVTG SREIKSQQSEVTRILDGKRI QYQLVDISQDNALRDEMRTL AGNPKATPPQIVNGNHYCGD YELFVEAVEQDTLQEFLKLA

Data sets:
Data typeCount
13C chemical shifts430
15N chemical shifts110
1H chemical shifts696

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1SH3 domain-binding glutamic acid-rich-like protein 31

Entities:

Entity 1, SH3 domain-binding glutamic acid-rich-like protein 3 100 residues - Formula weight is not available

1   GLYSERGLUGLYALAALATHRMETSERGLY
2   LEUARGVALTYRSERTHRSERVALTHRGLY
3   SERARGGLUILELYSSERGLNGLNSERGLU
4   VALTHRARGILELEUASPGLYLYSARGILE
5   GLNTYRGLNLEUVALASPILESERGLNASP
6   ASNALALEUARGASPGLUMETARGTHRLEU
7   ALAGLYASNPROLYSALATHRPROPROGLN
8   ILEVALASNGLYASNHISTYRCYSGLYASP
9   TYRGLULEUPHEVALGLUALAVALGLUGLN
10   ASPTHRLEUGLNGLUPHELEULYSLEUALA

Samples:

sample_1: SH3BGR, [U-13C; U-15N], 1.2 mM; phosphate 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1not availablecondition_1
3D 15N-separated NOESYsample_1not availablecondition_1
2D TROSY HNCOsample_1not availablecondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.613, Kobayashi, N. - data analysis

CYANA v1.0.6, Guntert, P. - refinement

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAC25034
GB AAH08110 AAI67002 EDL30032 EDL80707
REF NP_001100158 NP_542126
SP Q91VW3
AlphaFold Q91VW3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks