BMRB Entry 10096

Name: Backbone Resonance Assignments for LolB
Published: 2007-08-22

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10096

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone Resonance Assignments for LolB

Deposition date:

2007-01-31

Original release date:

2007-08-22

Authors:

Nakada, Shingo; Sakakura, Masayoshi; Takahashi, Hideo; Tokuda, Hajime; Shimada, Ichio

Citation:

Citation: Shingo, Nakada; Masayoshi, Sakakura; Hideo, Takahashi; Hajime, Tokuda; Ichio, Shimada. "Backbone resonance assignment for the outer membrane lipoprotein receptor LolB from Escherichia coli" Biomol. NMR Assignments 1, 121-123 (2007).
PubMed: 19636844

Assembly members:

Assembly members:
LolB, polymer, 187 residues, 21300 Da.

Natural source:

Natural source: Common Name: Escherichia coli K-12 Taxonomy ID: 83333 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
LolB: MASVTTPKGPGKSPDSPQWR QHQQDVRNLNQYQTRGAFAY ISDQQKVYARFFWQQTGQDR YRLLLTNPLGSTELELNAQP GNVQLVDNKGQRYTADDAEE MIGKLTGMPIPLNSLRQWIL GLPGDATDYKLDDQYRLSEI TYSQNGKNWKVVYGGYDTKT QPAMPANMELTDGGQRIKLK MDNWIVK

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	476
15N chemical shifts	159
1H chemical shifts	159

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	LolB monomer	1

Entities:

Entity 1, LolB monomer 187 residues - 21300 Da.

1

MET

ALA

SER

VAL

THR

PRO

LYS

GLY

PRO

2

GLY

LYS

SER

PRO

ASP

SER

PRO

GLN

TRP

ARG

3

GLN

HIS

GLN

ASP

VAL

ARG

ASN

LEU

ASN

4

GLN

TYR

GLN

THR

ARG

GLY

ALA

PHE

ALA

TYR

5

ILE

SER

ASP

GLN

LYS

VAL

TYR

ALA

ARG

6

PHE

TRP

GLN

THR

GLY

GLN

ASP

ARG

7

TYR

ARG

LEU

THR

ASN

PRO

LEU

GLY

8

SER

THR

GLU

LEU

GLU

LEU

ASN

ALA

GLN

PRO

9

GLY

ASN

VAL

GLN

LEU

VAL

ASP

ASN

LYS

GLY

10

GLN

ARG

TYR

THR

ALA

ASP

ALA

GLU

11

MET

ILE

GLY

LYS

LEU

THR

GLY

MET

PRO

ILE

12

PRO

LEU

ASN

SER

LEU

ARG

GLN

TRP

ILE

LEU

13

GLY

LEU

PRO

GLY

ASP

ALA

THR

ASP

TYR

LYS

14

LEU

ASP

GLN

TYR

ARG

LEU

SER

GLU

ILE

15

THR

TYR

SER

GLN

ASN

GLY

LYS

ASN

TRP

LYS

16

VAL

TYR

GLY

TYR

ASP

THR

LYS

THR

17

GLN

PRO

ALA

MET

PRO

ALA

ASN

MET

GLU

LEU

18

THR

ASP

GLY

GLN

ARG

ILE

LYS

LEU

LYS

19

MET

ASP

ASN

TRP

ILE

VAL

LYS

Samples:

sample_1: LolB, [U-2H; U-13C; U-15N], 0.5 – 1.0 mM; MES 38 mM; D2O 10%; H2O 90%

condition_1: pH: 6.9; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H15N TROSY-HSQC	sample_1	not available	condition_1
TROSY-HNCACB	sample_1	not available	condition_1
TROSY-HN(CO)CACB	sample_1	not available	condition_1
TROSY-HNCA	sample_1	not available	condition_1
TROSY-HN(CO)CA	sample_1	not available	condition_1
TROSY-HNCO	sample_1	not available	condition_1

Software:

xwinnmr v3.5, Bruker Japan - data collection

SPARKY v3.110, T. D. Goddard and D. G. Kneller - peak assignments

NMRPipe v2.3 - data processing

NMR spectrometers:

Bruker Avance600 600 MHz

PDB	1IWM 1IWN 3WJT 3WJU 3WJV
DBJ	BAA01105 BAA36067 BAB35137 BAG76783 BAI25021
EMBL	CAP75748 CAQ31711 CAQ89267 CAQ98089 CAR02603
GB	AAA24433 AAC74293 AAG56067 AAN42825 AAN80132
REF	NP_309741 NP_415727 NP_707118 WP_000174480 WP_001130605
SP	A7ZKY3 A7ZZE5 B1IU84 B1LH91 B1XAQ0
AlphaFold	A7ZKY3 A7ZZE5 B1IU84 B1LH91 B1XAQ0