BMRB Entry 10086

Name: RA domain of guanine nucleotide exchange factor for Rap1
Published: 2008-08-14

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PDB ID: 1wgy
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10086
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

RA domain of guanine nucleotide exchange factor for Rap1

Deposition date:

2007-01-24

Original release date:

2008-08-14

Authors:

Nagashima, T.; Tochio, N.; Kigawa, T.; Hayashi, F.; Yokoyama, S.

Citation:

Citation: Nagashima, T.; Tochio, N.; Kigawa, T.; Hayashi, F.; Yokoyama, S.. "RA domain of guanine nucleotide exchange factor for Rap1" .

Assembly members:

Assembly members:
RA domain, polymer, 104 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P030804-93

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RA domain: GSSGSSGEEIFCHVYITEHS YVSVKAKVSSIAQEILKVVA EKIQYAEEDLALVAITFSGE KHELQPNDLVISKSLEASGR IYVYRKDLADTLNPFAENSG PSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	420
15N chemical shifts	86
1H chemical shifts	684

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Rap guanine nucleotide exchange factor 5	1

Entities:

Entity 1, Rap guanine nucleotide exchange factor 5 104 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

GLU

ILE

2

PHE

CYS

HIS

VAL

TYR

ILE

THR

GLU

HIS

SER

3

TYR

VAL

SER

VAL

LYS

ALA

LYS

VAL

SER

4

ILE

ALA

GLN

GLU

ILE

LEU

LYS

VAL

ALA

5

GLU

LYS

ILE

GLN

TYR

ALA

GLU

ASP

LEU

6

ALA

LEU

VAL

ALA

ILE

THR

PHE

SER

GLY

GLU

7

LYS

HIS

GLU

LEU

GLN

PRO

ASN

ASP

LEU

VAL

8

ILE

SER

LYS

SER

LEU

GLU

ALA

SER

GLY

ARG

9

ILE

TYR

VAL

TYR

ARG

LYS

ASP

LEU

ALA

ASP

10

THR

LEU

ASN

PRO

PHE

ALA

GLU

ASN

SER

GLY

11

PRO

SER

GLY

Samples:

sample_1: RA domain, [U-13C; U-15N], 1.04 mM; d-TRIS 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.5; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	not available	condition_1
3D 15N-separated NOESY	sample_1	not available	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.8992, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	1WGY
DBJ	BAA13406 BAG09689 BAG58663
GB	EAW93745 EAW93746
REF	XP_011513954 XP_012303210
SP	Q92565
AlphaFold	Q92565