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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10082
MolProbity Validation Chart
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NMR-STAR v3 text file.
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Citation: He, F.; Muto, Y.; Inoue, M.; Kigawa, T.; Shirouzu, M.; Terada, T.; Yokoyama, S.. "Solution structure of RRM domain in calcipressin 1" .
Assembly members:
RRM domain, polymer, 104 residues, Formula weight is not available
Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: cell free synthesis Vector: P040113-35
Entity Sequences (FASTA):
RRM domain: GSSGSSGLIACVANDDVFSE
SETRAKFESLFRTYDKDTTF
QYFKSFKRVRINFSNPLSAA
DARLRLHKTEFLGKEMKLYF
AQTLHIGSSHLAPPNPDKSG
PSSG
Data type | Count |
13C chemical shifts | 448 |
15N chemical shifts | 91 |
1H chemical shifts | 670 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | calcipressin 1 | 1 |
Entity 1, calcipressin 1 104 residues - Formula weight is not available
1 | GLY | SER | SER | GLY | SER | SER | GLY | LEU | ILE | ALA | ||||
2 | CYS | VAL | ALA | ASN | ASP | ASP | VAL | PHE | SER | GLU | ||||
3 | SER | GLU | THR | ARG | ALA | LYS | PHE | GLU | SER | LEU | ||||
4 | PHE | ARG | THR | TYR | ASP | LYS | ASP | THR | THR | PHE | ||||
5 | GLN | TYR | PHE | LYS | SER | PHE | LYS | ARG | VAL | ARG | ||||
6 | ILE | ASN | PHE | SER | ASN | PRO | LEU | SER | ALA | ALA | ||||
7 | ASP | ALA | ARG | LEU | ARG | LEU | HIS | LYS | THR | GLU | ||||
8 | PHE | LEU | GLY | LYS | GLU | MET | LYS | LEU | TYR | PHE | ||||
9 | ALA | GLN | THR | LEU | HIS | ILE | GLY | SER | SER | HIS | ||||
10 | LEU | ALA | PRO | PRO | ASN | PRO | ASP | LYS | SER | GLY | ||||
11 | PRO | SER | SER | GLY |
sample_1: RRM domain, [U-13C; U-15N], 0.8 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
3D 15N-separated NOESY | sample_1 | not available | condition_1 |
3D 13C-separated NOESY | sample_1 | not available | condition_1 |
xwinnmr v2.6, Bruker - collection
NMRPipe v20020425, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.863, Kobayashi, N. - data analysis
CYANA v2.0.29, Guntert, P. - refinement, structure solution
PDB | |
DBJ | BAC36729 |
GB | AAF63486 AAF72701 AAH13551 EDL03783 |
REF | NP_062339 XP_012971691 |
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