BMRB Entry 10081

Name: Solution structure of the PDZ domain of mouse Rhophilin-2
Published: 2008-08-14

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PDB ID: 1vae
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10081
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the PDZ domain of mouse Rhophilin-2

Deposition date:

2007-01-23

Original release date:

2008-08-14

Authors:

Yoneyama, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Yoneyama, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the PDZ domain of mouse Rhophilin-2" .

Assembly members:

Assembly members:
PDZ domain, polymer, 111 residues, Formula weight is not available

Natural source:

Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P030203-40

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PDZ domain: GSSGSSGSASKRWSPPRGIH FTVEEGDLGFTLRGNTPVQV HFLDPHCSASLAGAKEGDYI VSIQGVDCKWLTVSEVMKLL KSFGGEEVEMKVVSLLDSTS SMHNKSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	427
15N chemical shifts	100
1H chemical shifts	678

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	rhophilin, Rho GTPase binding protein 2	1

Entities:

Entity 1, rhophilin, Rho GTPase binding protein 2 111 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

SER

ALA

SER

2

LYS

ARG

TRP

SER

PRO

ARG

GLY

ILE

HIS

3

PHE

THR

VAL

GLU

GLY

ASP

LEU

GLY

PHE

4

THR

LEU

ARG

GLY

ASN

THR

PRO

VAL

GLN

VAL

5

HIS

PHE

LEU

ASP

PRO

HIS

CYS

SER

ALA

SER

6

LEU

ALA

GLY

ALA

LYS

GLU

GLY

ASP

TYR

ILE

7

VAL

SER

ILE

GLN

GLY

VAL

ASP

CYS

LYS

TRP

8

LEU

THR

VAL

SER

GLU

VAL

MET

LYS

LEU

9

LYS

SER

PHE

GLY

GLU

VAL

GLU

MET

10

LYS

VAL

SER

LEU

ASP

SER

THR

SER

11

SER

MET

HIS

ASN

LYS

SER

GLY

PRO

SER

12

GLY

Samples:

sample_1: PDZ domain, [U-13C; U-15N], 1 mM; acetate 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 4.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	not available	condition_1
3D 13C-separated NOESY	sample_1	not available	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Jhonson, B.A. - data analysis

Kujira v0.891, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz