BMRB Entry 10078

Title:
Backbone Resonance Assignments for LolA
Deposition date:
2007-01-21
Original release date:
2007-08-22
Authors:
Nakada, Shingo; Takahashi, Hideo; Sakakura, Masayoshi; Kurono, Masuo; Shimada, Ichio
Citation:

Citation: Shingo, Nakada; Hideo, Takahashi; Masayoshi, Sakakura; Masuo, Kurono; Ichio, Shimada. "Backbone resonance assignments for the periplasmic chaperone LolA from Escherichia coli"  Biomol. NMR Assignments 1, 125-127 (2007).
PubMed: 19636845

Assembly members:

Assembly members:
LolA, polymer, 192 residues, 21600 Da.

Natural source:

Natural source:   Common Name: Escherichia coli K-12   Taxonomy ID: 83333   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts515
15N chemical shifts162
1H chemical shifts162

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1LolA monomer1

Entities:

Entity 1, LolA monomer 192 residues - 21600 Da.

1   METALAASPALAALASERASPLEULYSSER
2   ARGLEUASPLYSVALSERSERPHEHISALA
3   SERPHETHRGLNLYSVALTHRASPGLYSER
4   GLYALAALAVALGLNGLUGLYGLNGLYASP
5   LEUTRPVALLYSARGPROASNLEUPHEASN
6   TRPHISMETTHRGLNPROASPGLUSERILE
7   LEUVALSERASPGLYLYSTHRLEUTRPPHE
8   TYRASNPROPHEVALGLUGLNALATHRALA
9   THRTRPLEULYSASPALATHRGLYASNTHR
10   PROPHEMETLEUILEALAARGASNGLNSER
11   SERASPTRPGLNGLNTYRASNILELYSGLN
12   ASNGLYASPASPPHEVALLEUTHRPROLYS
13   ALASERASNGLYASNLEULYSGLNPHETHR
14   ILEASNVALGLYARGASPGLYTHRILEHIS
15   GLNPHESERALAVALGLUGLNASPASPGLN
16   ARGSERSERTYRGLNLEULYSSERGLNGLN
17   ASNGLYALAVALASPALAALALYSPHETHR
18   PHETHRPROPROGLNGLYVALTHRVALASP
19   ASPGLNARGLYSLEUGLUHISHISHISHIS
20   HISHIS

Samples:

sample_1: LolA, [U-2H; U-13C; U-15N], 0.5 – 1.0 mM; sodium phosphate 66 mM; sodium chloride 100 mM; D2O 10%; H2O 90%

condition_1: pH: 6.95; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
1H15N TROSY-HSQCsample_1not availablecondition_1
TROSY-HNCACBsample_1not availablecondition_1
TROSY-HN(CO)CACBsample_1not availablecondition_1
TROSY-HNCAsample_1not availablecondition_1
TROSY-HN(CO)CAsample_1not availablecondition_1
TROSY-HNCOsample_1not availablecondition_1
TROSY-HN(CA)COsample_1not availablecondition_1

Software:

xwinnmr v3.5, Bruker Japan - data collection

SPARKY v3.110, T. D. Goddard and D. G. Kneller - peak assignments

NMRPipe v2.3 - data processing

FELIX v2000 - data processing

NMR spectrometers:

  • Bruker Avance800 800 MHz

Related Database Links:

PDB
DBJ BAA08390 BAA35616 BAB34399 BAG76473 BAI24333
EMBL CAP75364 CAQ31419 CAQ88570 CAQ97795 CAR02254
GB AAC73977 AAG55378 AAN42483 AAN79500 AAP16355
PIR F85614 H90750
REF NP_309003 NP_415411 NP_706776 WP_000976047 WP_001245402
SP A7ZJW0 A7ZYJ5 B1IWN2 B1LJX2 B1X831
AlphaFold A7ZJW0 A7ZYJ5 B1IWN2 B1LJX2 B1X831

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks