BMRB Entry 10078

Name: Backbone Resonance Assignments for LolA
Published: 2007-08-22

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10078

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone Resonance Assignments for LolA

Deposition date:

2007-01-21

Original release date:

2007-08-22

Authors:

Nakada, Shingo; Takahashi, Hideo; Sakakura, Masayoshi; Kurono, Masuo; Shimada, Ichio

Citation:

Citation: Shingo, Nakada; Hideo, Takahashi; Masayoshi, Sakakura; Masuo, Kurono; Ichio, Shimada. "Backbone resonance assignments for the periplasmic chaperone LolA from Escherichia coli" Biomol. NMR Assignments 1, 125-127 (2007).
PubMed: 19636845

Assembly members:

Assembly members:
LolA, polymer, 192 residues, 21600 Da.

Natural source:

Natural source: Common Name: Escherichia coli K-12 Taxonomy ID: 83333 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
LolA: MADAASDLKSRLDKVSSFHA SFTQKVTDGSGAAVQEGQGD LWVKRPNLFNWHMTQPDESI LVSDGKTLWFYNPFVEQATA TWLKDATGNTPFMLIARNQS SDWQQYNIKQNGDDFVLTPK ASNGNLKQFTINVGRDGTIH QFSAVEQDDQRSSYQLKSQQ NGAVDAAKFTFTPPQGVTVD DQRKLEHHHHHH

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	515
15N chemical shifts	162
1H chemical shifts	162

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	LolA monomer	1

Entities:

Entity 1, LolA monomer 192 residues - 21600 Da.

1

MET

ALA

ASP

ALA

SER

ASP

LEU

LYS

SER

2

ARG

LEU

ASP

LYS

VAL

SER

PHE

HIS

ALA

3

SER

PHE

THR

GLN

LYS

VAL

THR

ASP

GLY

SER

4

GLY

ALA

VAL

GLN

GLU

GLY

GLN

GLY

ASP

5

LEU

TRP

VAL

LYS

ARG

PRO

ASN

LEU

PHE

ASN

6

TRP

HIS

MET

THR

GLN

PRO

ASP

GLU

SER

ILE

7

LEU

VAL

SER

ASP

GLY

LYS

THR

LEU

TRP

PHE

8

TYR

ASN

PRO

PHE

VAL

GLU

GLN

ALA

THR

ALA

9

THR

TRP

LEU

LYS

ASP

ALA

THR

GLY

ASN

THR

10

PRO

PHE

MET

LEU

ILE

ALA

ARG

ASN

GLN

SER

11

SER

ASP

TRP

GLN

TYR

ASN

ILE

LYS

GLN

12

ASN

GLY

ASP

PHE

VAL

LEU

THR

PRO

LYS

13

ALA

SER

ASN

GLY

ASN

LEU

LYS

GLN

PHE

THR

14

ILE

ASN

VAL

GLY

ARG

ASP

GLY

THR

ILE

HIS

15

GLN

PHE

SER

ALA

VAL

GLU

GLN

ASP

GLN

16

ARG

SER

TYR

GLN

LEU

LYS

SER

GLN

17

ASN

GLY

ALA

VAL

ASP

ALA

LYS

PHE

THR

18

PHE

THR

PRO

GLN

GLY

VAL

THR

VAL

ASP

19

ASP

GLN

ARG

LYS

LEU

GLU

HIS

20

HIS

Samples:

sample_1: LolA, [U-2H; U-13C; U-15N], 0.5 – 1.0 mM; sodium phosphate 66 mM; sodium chloride 100 mM; D2O 10%; H2O 90%

condition_1: pH: 6.95; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H15N TROSY-HSQC	sample_1	not available	condition_1
TROSY-HNCACB	sample_1	not available	condition_1
TROSY-HN(CO)CACB	sample_1	not available	condition_1
TROSY-HNCA	sample_1	not available	condition_1
TROSY-HN(CO)CA	sample_1	not available	condition_1
TROSY-HNCO	sample_1	not available	condition_1
TROSY-HN(CA)CO	sample_1	not available	condition_1

Software:

xwinnmr v3.5, Bruker Japan - data collection

SPARKY v3.110, T. D. Goddard and D. G. Kneller - peak assignments

NMRPipe v2.3 - data processing

FELIX v2000 - data processing

NMR spectrometers:

Bruker Avance800 800 MHz

PDB	1IWL 1UA8 2ZPC 2ZPD 3KSN
DBJ	BAA08390 BAA35616 BAB34399 BAG76473 BAI24333
EMBL	CAP75364 CAQ31419 CAQ88570 CAQ97795 CAR02254
GB	AAC73977 AAG55378 AAN42483 AAN79500 AAP16355
PIR	F85614 H90750
REF	NP_309003 NP_415411 NP_706776 WP_000976047 WP_001245402
SP	A7ZJW0 A7ZYJ5 B1IWN2 B1LJX2 B1X831
AlphaFold	A7ZJW0 A7ZYJ5 B1IWN2 B1LJX2 B1X831