BMRB Entry 10077

Name: Solution structure of the RNA binding domain of eukaryotic initiation factor 4B
Published: 2008-08-14

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PDB ID: 1wi8
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10077
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the RNA binding domain of eukaryotic initiation factor 4B

Deposition date:

2006-12-25

Original release date:

2008-08-14

Authors:

Suzuki, S.; Muto, Y.; Nagata, T.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.

Citation:

Citation: Suzuki, S.; Muto, Y.; Nagata, T.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.. "Solution structure of the RNA binding domain of eukaryotic initiation factor 4B" .

Assembly members:

Assembly members:
RNA recognition motif, polymer, 104 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P040301-18

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RNA recognition motif: GSSGSSGSRLPKSPPYTAFL GNLPYDVTEESIKEFFRGLN ISAVRLPREPSNPERLKGFG YAEFEDLDSLLSALSLNEES LGNKRIRVDVADQAQDKDSG PSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	415
15N chemical shifts	93
1H chemical shifts	647

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Eukaryotic translation initiation factor 4B	1

Entities:

Entity 1, Eukaryotic translation initiation factor 4B 104 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

SER

ARG

LEU

2

PRO

LYS

SER

PRO

TYR

THR

ALA

PHE

LEU

3

GLY

ASN

LEU

PRO

TYR

ASP

VAL

THR

GLU

4

SER

ILE

LYS

GLU

PHE

ARG

GLY

LEU

ASN

5

ILE

SER

ALA

VAL

ARG

LEU

PRO

ARG

GLU

PRO

6

SER

ASN

PRO

GLU

ARG

LEU

LYS

GLY

PHE

GLY

7

TYR

ALA

GLU

PHE

GLU

ASP

LEU

ASP

SER

LEU

8

LEU

SER

ALA

LEU

SER

LEU

ASN

GLU

SER

9

LEU

GLY

ASN

LYS

ARG

ILE

ARG

VAL

ASP

VAL

10

ALA

ASP

GLN

ALA

GLN

ASP

LYS

ASP

SER

GLY

11

PRO

SER

GLY

Samples:

sample_1: RNA recognition motif, [U-13C; U-15N], 1.0 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	not available	condition_1
3D 15N-separated NOESY	sample_1	not available	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v2.3, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.901, Kobayashi, N. - data analysis

Olivia v1.9.12, Yokochi, M. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	1WI8 2J76
DBJ	BAB82380 BAD96248 BAG11107 BAG57157 BAG61335
EMBL	CAA39265 CAG33239 CAH92323
GB	AAH73139 AAH73154 AAH98437 AAI05158 AIC54329
REF	NP_001030200 NP_001182737 NP_001287750 NP_001408 XP_001115324
SP	P23588
TPG	DAA30015
AlphaFold	P23588