BMRB Entry 10076

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10076
MolProbity Validation Chart

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Title:
The eighth FN3 domain of human sidekick-2
Deposition date:
2006-12-25
Original release date:
2008-08-14
Authors:
Nagashima, T.; Hayashi, F.; Yokoyama, S.
Citation:

Citation: Nagashima, T.; Hayashi, F.; Yokoyama, S.. "The eighth FN3 domain of human sidekick-2"  .

Assembly members:

Assembly members:
FN3 domain, polymer, 130 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P040223-52

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FN3 domain: GSSGSSGRIGDGSPSHPPIL ERTLDDVPGPPMGILFPEVR TTSVRLIWQPPAAPNGIILA YQITHRLNTTTANTATVEVL APSARQYTATGLKPESVYLF RITAQTRKGWGEAAEALVVT TEKRSGPSSG

Data sets:
Data typeCount
13C chemical shifts546
15N chemical shifts123
1H chemical shifts866

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1sidekick 21

Entities:

Entity 1, sidekick 2 130 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYARGILEGLY
2   ASPGLYSERPROSERHISPROPROILELEU
3   GLUARGTHRLEUASPASPVALPROGLYPRO
4   PROMETGLYILELEUPHEPROGLUVALARG
5   THRTHRSERVALARGLEUILETRPGLNPRO
6   PROALAALAPROASNGLYILEILELEUALA
7   TYRGLNILETHRHISARGLEUASNTHRTHR
8   THRALAASNTHRALATHRVALGLUVALLEU
9   ALAPROSERALAARGGLNTYRTHRALATHR
10   GLYLEULYSPROGLUSERVALTYRLEUPHE
11   ARGILETHRALAGLNTHRARGLYSGLYTRP
12   GLYGLUALAALAGLUALALEUVALVALTHR
13   THRGLULYSARGSERGLYPROSERSERGLY

Samples:

sample_1: FN3 domain, [U-13C; U-15N], 1.00 mM; d-TRIS 20 mM; NaCl 200 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 220 mM; pH: 7.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1not availablecondition_1
3D 15N-separated NOESYsample_1not availablecondition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.8992, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement", structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB
REF XP_009189425

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks