BMRB Entry 10069

Title:
Solution structure of the Villin headpiece domain of human actin-binding LIM protein homologue (KIAA0843 protein)
Deposition date:
2006-12-25
Original release date:
2008-08-14
Authors:
Tochio, N.; Koshiba, S.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Tochio, N.; Koshiba, S.; Kigawa, T.; Yokoyama, S.. "Solution structure of the Villin headpiece domain of human actin-binding LIM protein homologue (KIAA0843 protein)"  .

Assembly members:

Assembly members:
VHP domain, polymer, 88 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P020930-61

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts402
15N chemical shifts98
1H chemical shifts620

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1actin-binding LIM protein homologue1

Entities:

Entity 1, actin-binding LIM protein homologue 88 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYASNALAVAL
2   ASNTRPGLYMETARGGLUTYRLYSILETYR
3   PROTYRGLULEULEULEUVALTHRTHRARG
4   GLYARGASNARGLEUPROLYSASPVALASP
5   ARGTHRARGLEUGLUARGHISLEUSERGLN
6   GLUGLUPHETYRGLNVALPHEGLYMETTHR
7   ILESERGLUPHEASPARGLEUALALEUTRP
8   LYSARGASNGLULEULYSLYSGLNALAARG
9   LEUPHESERGLYPROSERSERGLY

Samples:

sample_1: VHP domain, [U-13C; U-15N], 1.2 mM; phosphate 20 mM; NaCl 100 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1not availablecondition_1
3D 15N-separated NOESYsample_1not availablecondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v200204025, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.816, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAA74866 BAD32318 BAD38663 BAF82425 BAG09865
EMBL CAH56270 CAH92935 CAL37473 CAL37581 CAL37743
GB AAH60275 AAH94229 AAI42520 ABD83327 EAW61788
REF NP_001092597 NP_001126727 NP_001157963 NP_001178627 NP_001287944
SP O94929 Q69ZX8
TPG DAA27290
AlphaFold O94929 Q69ZX8

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks