BMRB Entry 10069

Click here to enlarge.

PDB ID: 1ujs
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10069
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Solution structure of the Villin headpiece domain of human actin-binding LIM protein homologue (KIAA0843 protein)

Deposition date:

2006-12-25

Original release date:

2008-08-14

Authors:

Tochio, N.; Koshiba, S.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Tochio, N.; Koshiba, S.; Kigawa, T.; Yokoyama, S.. "Solution structure of the Villin headpiece domain of human actin-binding LIM protein homologue (KIAA0843 protein)" .

Assembly members:

Assembly members:
VHP domain, polymer, 88 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P020930-61

Entity Sequences (FASTA):

Entity Sequences (FASTA):
VHP domain: GSSGSSGNAVNWGMREYKIY PYELLLVTTRGRNRLPKDVD RTRLERHLSQEEFYQVFGMT ISEFDRLALWKRNELKKQAR LFSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	402
15N chemical shifts	98
1H chemical shifts	620

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	actin-binding LIM protein homologue	1

Entities:

Entity 1, actin-binding LIM protein homologue 88 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ASN

ALA

VAL

2

ASN

TRP

GLY

MET

ARG

GLU

TYR

LYS

ILE

TYR

3

PRO

TYR

GLU

LEU

VAL

THR

ARG

4

GLY

ARG

ASN

ARG

LEU

PRO

LYS

ASP

VAL

ASP

5

ARG

THR

ARG

LEU

GLU

ARG

HIS

LEU

SER

GLN

6

GLU

PHE

TYR

GLN

VAL

PHE

GLY

MET

THR

7

ILE

SER

GLU

PHE

ASP

ARG

LEU

ALA

LEU

TRP

8

LYS

ARG

ASN

GLU

LEU

LYS

GLN

ALA

ARG

9

LEU

PHE

SER

GLY

PRO

SER

GLY

Samples:

sample_1: VHP domain, [U-13C; U-15N], 1.2 mM; phosphate 20 mM; NaCl 100 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	not available	condition_1
3D 15N-separated NOESY	sample_1	not available	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v200204025, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.816, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	1UJS
DBJ	BAA74866 BAD32318 BAD38663 BAF82425 BAG09865
EMBL	CAH56270 CAH92935 CAL37473 CAL37581 CAL37743
GB	AAH60275 AAH94229 AAI42520 ABD83327 EAW61788
REF	NP_001092597 NP_001126727 NP_001157963 NP_001178627 NP_001287944
SP	O94929 Q69ZX8
TPG	DAA27290
AlphaFold	O94929 Q69ZX8