BMRB Entry 10067

Name: Solution Structure of The FHA Domain of Arabidopsis thaliana Hypothetical Protein
Published: 2008-08-14

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PDB ID: 1uht
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10067
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of The FHA Domain of Arabidopsis thaliana Hypothetical Protein

Deposition date:

2006-12-25

Original release date:

2008-08-14

Authors:

Tomizawa, T.; Inoue, M.; Koshiba, S.; Hayashi, F.; Shirouzu, M.; Terada, T.; Yabuki, T.; Aoki, M.; Matsuda, T.; Seki, E.; Hirota, H.; Yoshida, M.; Tanaka, A.; Osanai, T.; Shinozaki, K.; Seki, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Tomizawa, T.; Inoue, M.; Koshiba, S.; Hayashi, F.; Shirouzu, M.; Terada, T.; Yabuki, T.; Aoki, M.; Matsuda, T.; Seki, E.; Hirota, H.; Yoshida, M.; Tanaka, A.; Osanai, T.; Shinozaki, K.; Seki, M.; Kigawa, T.; Yokoyama, S.. "Solution Structure of The FHA Domain of Arabidopsis thaliana Hypothetical Protein" .

Assembly members:

Assembly members:
Forkhead Associated Domain, FHA domain, polymer, 118 residues, Formula weight is not available

Natural source:

Natural source: Common Name: thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P021218-71

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Forkhead Associated Domain, FHA domain: GSSGSSGMVTPSLRLVFVKG PREGDALDYKPGSTIRVGRI VRGNEIAIKDAGISTKHLRI ESDSGNWVIQDLGSSNGTLL NSNALDPETSVNLGDGDVIK LGEYTSILVNFVSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	473
15N chemical shifts	113
1H chemical shifts	761

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	expressed protein	1

Entities:

Entity 1, expressed protein 118 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

MET

VAL

THR

2

PRO

SER

LEU

ARG

LEU

VAL

PHE

VAL

LYS

GLY

3

PRO

ARG

GLU

GLY

ASP

ALA

LEU

ASP

TYR

LYS

4

PRO

GLY

SER

THR

ILE

ARG

VAL

GLY

ARG

ILE

5

VAL

ARG

GLY

ASN

GLU

ILE

ALA

ILE

LYS

ASP

6

ALA

GLY

ILE

SER

THR

LYS

HIS

LEU

ARG

ILE

7

GLU

SER

ASP

SER

GLY

ASN

TRP

VAL

ILE

GLN

8

ASP

LEU

GLY

SER

ASN

GLY

THR

LEU

9

ASN

SER

ASN

ALA

LEU

ASP

PRO

GLU

THR

SER

10

VAL

ASN

LEU

GLY

ASP

GLY

ASP

VAL

ILE

LYS

11

LEU

GLY

GLU

TYR

THR

SER

ILE

LEU

VAL

ASN

12

PHE

VAL

SER

GLY

PRO

SER

GLY

Samples:

sample_1: FHA domain, [U-13C; U-15N], 1.09 mM; Tris-HCl 20 mM; NaCl 100 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	not available	not available	not available
3D 15N-separated NOESY	not available	not available	not available

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.820, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

Varian INOVA 800 MHz

PDB	1UHT
EMBL	CAB10228 CAB78491
GB	AAM20599 AAM91265 AEE83450
REF	NP_193185
SP	O23305
AlphaFold	O23305