BMRB Entry 10062

Title:
Backbone 1H and 15N Chemical Shift Assignments for Nedd8
Deposition date:
2006-12-07
Original release date:
2007-03-23
Authors:
Sakata, Eri; Yamaguchi, Yoshiki; Miyauchi, Yasuhiro; Iwai, Kazuhiro; Chiba, Tomoki; Saeki, Yasusi; Matsuda, Noriyuki; Tanaka, Keiji; Kato, Koichi
Citation:

Citation: Sakata, Eri; Yamaguchi, Yoshiki; Miyauchi, Yasuhiro; Iwai, Kazuhiro; Chiba, Tomoki; Saeki, Yasusi; Matsuda, Noriyuki; Tanaka, Keiji; Kato, Koichi. "Direct interactions between Nedd8 and ubiquitin E2 conjugating enzymes upregulate cullin-based E3 ligase activity"  Nat. Struct. Mol. Biol. 14, 167-168 (2007).
PubMed: 17206147

Assembly members:

Assembly members:
Nedd8, polymer, 96 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
  • assigned_chemical_shifts
Data typeCount
15N chemical shifts72
1H chemical shifts72

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Nedd8 monomer1

Entities:

Entity 1, Nedd8 monomer 96 residues - Formula weight is not available

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METLEUILELYSVALLYSTHRLEUTHRGLY
4   LYSGLUILEGLUILEASPILEGLUPROTHR
5   ASPLYSVALGLUARGILELYSGLUARGVAL
6   GLUGLULYSGLUGLYILEPROPROGLNGLN
7   GLNARGLEUILETYRSERGLYLYSGLNMET
8   ASNASPGLULYSTHRALAALAASPTYRLYS
9   ILELEUGLYGLYSERVALLEUHISLEUVAL
10   LEUALALEUARGGLYGLY

Samples:

sample_1: Nedd8, [U-98% 13C; U-98% 15N], 1 mM; MES 50 mM

sample_condition_1: pH: 6.0; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Bruker DMX500 500 MHz

Related Database Links:

PDB
DBJ BAA01719 BAA04889 BAE27394 BAE27660 BAE34759
EMBL CAF89342 CAF97715 CAG05755 CAG28590 CAJ82981
GB AAC64189 AAF73911 AAH04625 AAH55645 AAH76245
REF NP_001002557 NP_001016973 NP_001075681 NP_001154087 NP_001187300
SP P29595 P61282 Q15843 Q4PLJ0 Q71UE8
TPG DAA25711
AlphaFold P29595 P61282 Q15843 Q4PLJ0 Q71UE8

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks