BMRB Entry 10058

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PDB ID: 1wik
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10058
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of the PICOT homology 2 domain of the mouse PKC-interacting cousin of thioredoxin protein

Deposition date:

2006-11-29

Original release date:

2008-08-13

Authors:

Miyamoto, K.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Miyamoto, K.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the PICOT homology 2 domain of the mouse PKC-interacting cousin of thioredoxin protein" .

Assembly members:

Assembly members:
PICOT homology 2 domain, polymer, 109 residues, Formula weight is not available

Natural source:

Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P030421-03

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PICOT homology 2 domain: GSSGSSGLKVLTNKASVMLF MKGNKQEAKCGFSKQILEIL NSTGVEYETFDILEDEEVRQ GLKTFSNWPTYPQLYVRGDL VGGLDIVKELKDNGELLPIL KGESGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	452
15N chemical shifts	101
1H chemical shifts	724

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Thioredoxin-like protein 2	1

Entities:

Entity 1, Thioredoxin-like protein 2 109 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

LEU

LYS

VAL

2

LEU

THR

ASN

LYS

ALA

SER

VAL

MET

LEU

PHE

3

MET

LYS

GLY

ASN

LYS

GLN

GLU

ALA

LYS

CYS

4

GLY

PHE

SER

LYS

GLN

ILE

LEU

GLU

ILE

LEU

5

ASN

SER

THR

GLY

VAL

GLU

TYR

GLU

THR

PHE

6

ASP

ILE

LEU

GLU

ASP

GLU

VAL

ARG

GLN

7

GLY

LEU

LYS

THR

PHE

SER

ASN

TRP

PRO

THR

8

TYR

PRO

GLN

LEU

TYR

VAL

ARG

GLY

ASP

LEU

9

VAL

GLY

LEU

ASP

ILE

VAL

LYS

GLU

LEU

10

LYS

ASP

ASN

GLY

GLU

LEU

PRO

ILE

LEU

11

LYS

GLY

GLU

SER

GLY

PRO

SER

GLY

Samples:

sample_1: PICOT homology 2 domain, [U-13C; U-15N], 0.65 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	not available	condition_1
3D 15N-separated NOESY	sample_1	not available	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.8996, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 600 MHz