BMRB Entry 10057

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10057
MolProbity Validation Chart

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Title:
Solution structure of Iron-sulfur cluster protein U (IscU)
Deposition date:
2006-11-29
Original release date:
2008-08-13
Authors:
Nakanishi, T.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Nakanishi, T.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of Iron-sulfur cluster protein U (IscU)"  .

Assembly members:

Assembly members:
Iron-sulfur cluster protein U (IscU), polymer, 130 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P020826-02

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Iron-sulfur cluster protein U (IscU): GSSGSSGENPRNVGSLDKTS KNVGTGLVGAPACGDVMKLQ IQVDEKGKIVDARFKTFGCG SAIASSSLATEWVKGKTVEE ALTIKNTDIAKELCLPPVKL HCSMLAEDAIKAALADYKLK QESKSGPSSG

Data sets:
Data typeCount
13C chemical shifts508
15N chemical shifts124
1H chemical shifts852

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1nitrogen fixation cluster-like1
2ZINC (II) ION2

Entities:

Entity 1, nitrogen fixation cluster-like 130 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYGLUASNPRO
2   ARGASNVALGLYSERLEUASPLYSTHRSER
3   LYSASNVALGLYTHRGLYLEUVALGLYALA
4   PROALACYSGLYASPVALMETLYSLEUGLN
5   ILEGLNVALASPGLULYSGLYLYSILEVAL
6   ASPALAARGPHELYSTHRPHEGLYCYSGLY
7   SERALAILEALASERSERSERLEUALATHR
8   GLUTRPVALLYSGLYLYSTHRVALGLUGLU
9   ALALEUTHRILELYSASNTHRASPILEALA
10   LYSGLULEUCYSLEUPROPROVALLYSLEU
11   HISCYSSERMETLEUALAGLUASPALAILE
12   LYSALAALALEUALAASPTYRLYSLEULYS
13   GLNGLUSERLYSSERGLYPROSERSERGLY

Entity 2, ZINC (II) ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: IscU domain, [U-13C; U-15N], 1.12 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 10 mM; NaN3 0.02%; ZnCl2 0.01 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1not availablecondition_1
3D 13C-separated NOESYsample_1not availablecondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.870, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement", structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAB26031 BAC30464 BAC38830 BAE91311 BAG51892
EMBL CAL38012
GB AAC50885 AAG37427 AAG37428 AAH11906 AAH28800
REF NP_001069151 NP_001099406 NP_001230245 NP_001248403 NP_001270426
SP Q9D7P6 Q9H1K1
TPG DAA20564
AlphaFold Q9D7P6 Q9H1K1

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks