BMRB Entry 10056

Name: Solution structure of the Ras-binding domain of mouse RGS14
Published: 2008-08-13

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PDB ID: 1wfy
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10056
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the Ras-binding domain of mouse RGS14

Deposition date:

2006-11-29

Original release date:

2008-08-13

Authors:

Nakanishi, T.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Nakanishi, T.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the Ras-binding domain of mouse RGS14" .

Assembly members:

Assembly members:
Raf-like Ras-binding domain, polymer, 104 residues, Formula weight is not available

Natural source:

Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P030120-12

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Raf-like Ras-binding domain: GSSGSSGDQEVRLENRITFQ LELVGLERVVRISAKPTKRL QEALQPILAKHGLSLDQVVL HRPGEKQPMDLENPVSSVAS QTLVLDTPPDAKMSEARSSG PSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	410
15N chemical shifts	88
1H chemical shifts	675

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	regulator of G-protein signaling 14; rap1/rap2 interacting protein	1

Entities:

Entity 1, regulator of G-protein signaling 14; rap1/rap2 interacting protein 104 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ASP

GLN

GLU

2

VAL

ARG

LEU

GLU

ASN

ARG

ILE

THR

PHE

GLN

3

LEU

GLU

LEU

VAL

GLY

LEU

GLU

ARG

VAL

4

ARG

ILE

SER

ALA

LYS

PRO

THR

LYS

ARG

LEU

5

GLN

GLU

ALA

LEU

GLN

PRO

ILE

LEU

ALA

LYS

6

HIS

GLY

LEU

SER

LEU

ASP

GLN

VAL

LEU

7

HIS

ARG

PRO

GLY

GLU

LYS

GLN

PRO

MET

ASP

8

LEU

GLU

ASN

PRO

VAL

SER

VAL

ALA

SER

9

GLN

THR

LEU

VAL

LEU

ASP

THR

PRO

ASP

10

ALA

LYS

MET

SER

GLU

ALA

ARG

SER

GLY

11

PRO

SER

GLY

Samples:

sample_1: RBD, [U-13C; U-15N], 0.18 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	not available	condition_1
3D 15N-separated NOESY	sample_1	not available	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.870, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 600 MHz