BMRB Entry 10046

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PDB ID: 1wi0
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10046
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the PB1 domain of mouse mitogen activated protein kinase kinase 5 (MAP2K5)

Deposition date:

2006-11-09

Original release date:

2008-08-13

Authors:

Yoneyama, M.; Hayashi, F.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Yoneyama, M.; Hayashi, F.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the PB1 domain of mouse mitogen activated protein kinase kinase 5 (MAP2K5)" .

Assembly members:

Assembly members:
PB1 domain, polymer, 113 residues, Formula weight is not available

Natural source:

Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P031125-28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PB1 domain: GSSGSSGPFCAMENQVLVIR IKIPNSGAVDWTVHSGPQLL FRDVLDVIGQVLPEATTTAF EYEDEDGDRITVRSDEEMKA MLSYYYSTVMEQQVNGQLIE PLQIFPRSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	473
15N chemical shifts	108
1H chemical shifts	743

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Mitogen activated protein kinase kinase 5	1

Entities:

Entity 1, Mitogen activated protein kinase kinase 5 113 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

PRO

PHE

CYS

2

ALA

MET

GLU

ASN

GLN

VAL

LEU

VAL

ILE

ARG

3

ILE

LYS

ILE

PRO

ASN

SER

GLY

ALA

VAL

ASP

4

TRP

THR

VAL

HIS

SER

GLY

PRO

GLN

LEU

5

PHE

ARG

ASP

VAL

LEU

ASP

VAL

ILE

GLY

GLN

6

VAL

LEU

PRO

GLU

ALA

THR

ALA

PHE

7

GLU

TYR

GLU

ASP

GLU

ASP

GLY

ASP

ARG

ILE

8

THR

VAL

ARG

SER

ASP

GLU

MET

LYS

ALA

9

MET

LEU

SER

TYR

SER

THR

VAL

MET

10

GLU

GLN

VAL

ASN

GLY

GLN

LEU

ILE

GLU

11

PRO

LEU

GLN

ILE

PHE

PRO

ARG

SER

GLY

PRO

12

SER

GLY

Samples:

sample_1: PB1 domain, [U-13C; U-15N], 1 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 297 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	not available	condition_1
3D 13C-separated NOESY	sample_1	not available	condition_1

Software:

VNMR v6.1c, Varian - collection

NMRView v5.0.4, Jhonson, B.A. - data analysis

Kujira v0.896, Kobayashi, N. - data analysis

CYANA v1.0.8, Guntert, P. - refinement, structure solution

NMRPipe v20020425, Delaglio, F. - processing

NMR spectrometers:

Varian INOVA 800 MHz

PDB	1WI0
DBJ	BAB32187
GB	EDL26038 EDL95771 EGW04324 ELK05300 ELW62370