BMRB Entry 10040

Name: Solution Structure of the RA Domain of Human Grb7 Protein
Published: 2008-08-13

Click here to enlarge.

PDB ID: 1wgr
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10040
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Solution Structure of the RA Domain of Human Grb7 Protein

Deposition date:

2006-11-09

Original release date:

2008-08-13

Authors:

Li, H.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Li, H.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the RA Domain of Human Grb7 Protein" .

Assembly members:

Assembly members:
RA domain, polymer, 100 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P040223-63

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RA domain: GSSGSSGRPHVVKVYSEDGA CRSVEVAAGATARHVCEMLV QRAHALSDETWGLVECHPHL ALERGLEDHESVVEVQAAWP VGGDSRFVFRKNFASGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	392
15N chemical shifts	95
1H chemical shifts	609

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Growth factor receptor-bound protein 7	1

Entities:

Entity 1, Growth factor receptor-bound protein 7 100 residues - Formula weight is not available

1	GLY	SER	SER	GLY	SER	SER	GLY	ARG	PRO	HIS
2	VAL	VAL	LYS	VAL	TYR	SER	GLU	ASP	GLY	ALA
3	CYS	ARG	SER	VAL	GLU	VAL	ALA	ALA	GLY	ALA
4	THR	ALA	ARG	HIS	VAL	CYS	GLU	MET	LEU	VAL
5	GLN	ARG	ALA	HIS	ALA	LEU	SER	ASP	GLU	THR
6	TRP	GLY	LEU	VAL	GLU	CYS	HIS	PRO	HIS	LEU
7	ALA	LEU	GLU	ARG	GLY	LEU	GLU	ASP	HIS	GLU
8	SER	VAL	VAL	GLU	VAL	GLN	ALA	ALA	TRP	PRO
9	VAL	GLY	GLY	ASP	SER	ARG	PHE	VAL	PHE	ARG
10	LYS	ASN	PHE	ALA	SER	GLY	PRO	SER	SER	GLY

Samples:

sample_1: RA domain, [U-13C, U-15N], 1.17 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	not available	condition_1
3D 15N-separated NOESY	sample_1	not available	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.897, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	1WGR
DBJ	BAA07827 BAA13412 BAA29059 BAA29060 BAD96569
GB	AAG25938 AAH06535 AAP35332 AAP36681 AAX29563
REF	NP_001025173 NP_001229371 NP_001229372 NP_005301 XP_001171807
SP	Q14451
AlphaFold	Q14451

1	GLY	SER	SER	GLY	SER	SER	GLY	ARG	PRO	HIS
2	VAL	VAL	LYS	VAL	TYR	SER	GLU	ASP	GLY	ALA
3	CYS	ARG	SER	VAL	GLU	VAL	ALA	ALA	GLY	ALA
4	THR	ALA	ARG	HIS	VAL	CYS	GLU	MET	LEU	VAL
5	GLN	ARG	ALA	HIS	ALA	LEU	SER	ASP	GLU	THR
6	TRP	GLY	LEU	VAL	GLU	CYS	HIS	PRO	HIS	LEU
7	ALA	LEU	GLU	ARG	GLY	LEU	GLU	ASP	HIS	GLU
8	SER	VAL	VAL	GLU	VAL	GLN	ALA	ALA	TRP	PRO
9	VAL	GLY	GLY	ASP	SER	ARG	PHE	VAL	PHE	ARG
10	LYS	ASN	PHE	ALA	SER	GLY	PRO	SER	SER	GLY

1	GLY	SER	SER	GLY	SER	SER	GLY	ARG	PRO	HIS
2	VAL	VAL	LYS	VAL	TYR	SER	GLU	ASP	GLY	ALA
3	CYS	ARG	SER	VAL	GLU	VAL	ALA	ALA	GLY	ALA
4	THR	ALA	ARG	HIS	VAL	CYS	GLU	MET	LEU	VAL
5	GLN	ARG	ALA	HIS	ALA	LEU	SER	ASP	GLU	THR
6	TRP	GLY	LEU	VAL	GLU	CYS	HIS	PRO	HIS	LEU
7	ALA	LEU	GLU	ARG	GLY	LEU	GLU	ASP	HIS	GLU
8	SER	VAL	VAL	GLU	VAL	GLN	ALA	ALA	TRP	PRO
9	VAL	GLY	GLY	ASP	SER	ARG	PHE	VAL	PHE	ARG
10	LYS	ASN	PHE	ALA	SER	GLY	PRO	SER	SER	GLY