BMRB Entry 10039

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PDB ID: 1wgq
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10039
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of the Pleckstrin Homology Domain of Mouse Ethanol Decreased 4 Protein

Deposition date:

2006-11-09

Original release date:

2008-08-13

Authors:

Li, H.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Li, H.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the Pleckstrin Homology Domain of Mouse Ethanol Decreased 4 Protein" .

Assembly members:

Assembly members:
Pleckstrin Homology domain, polymer, 109 residues, Formula weight is not available

Natural source:

Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P030212-46

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Pleckstrin Homology domain: GSSGSSGSTMSGYLYRSKGS KKPWKHLWFVIKNKVLYTYA ASEDVAALESQPLLGFTVTL VKDENSESKVFQLLHKGMVF YVFKADDAHSTQRWIDAFQE GTVSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	489
15N chemical shifts	112
1H chemical shifts	744

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FYVE, RhoGEF and PH domain containing 6; ethanol decreased 4	1

Entities:

Entity 1, FYVE, RhoGEF and PH domain containing 6; ethanol decreased 4 109 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

SER

THR

MET

2

SER

GLY

TYR

LEU

TYR

ARG

SER

LYS

GLY

SER

3

LYS

PRO

TRP

LYS

HIS

LEU

TRP

PHE

VAL

4

ILE

LYS

ASN

LYS

VAL

LEU

TYR

THR

TYR

ALA

5

ALA

SER

GLU

ASP

VAL

ALA

LEU

GLU

SER

6

GLN

PRO

LEU

GLY

PHE

THR

VAL

THR

LEU

7

VAL

LYS

ASP

GLU

ASN

SER

GLU

SER

LYS

VAL

8

PHE

GLN

LEU

HIS

LYS

GLY

MET

VAL

PHE

9

TYR

VAL

PHE

LYS

ALA

ASP

ALA

HIS

SER

10

THR

GLN

ARG

TRP

ILE

ASP

ALA

PHE

GLN

GLU

11

GLY

THR

VAL

SER

GLY

PRO

SER

GLY

Samples:

sample_1: Pleckstrin Homology domain, [U-13C, U-15N], 1.22 mM; Phosphate 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	not available	condition_1
3D 15N-separated NOESY	sample_1	not available	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.897, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 600 MHz