BMRB Entry 10038

Title:
Solution structure of zf-C2H2 domains from human Zinc finger protein 295
Deposition date:
2006-11-02
Original release date:
2008-08-12
Authors:
Tomizawa, T.; Kigawa, T.; Saito, K.; Koshiba, S.; Inoue, M.; Yokoyama, S.
Citation:

Citation: Tomizawa, T.; Kigawa, T.; Saito, K.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of zf-C2H2 domains from human Zinc finger protein 295"  .

Assembly members:

Assembly members:
zf-C2H2 domain, polymer, 107 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P040126-28

Data sets:
Data typeCount
13C chemical shifts447
15N chemical shifts106
1H chemical shifts689

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Zinc finger protein 2951
2ZINC (II) ION, 12
3ZINC (II) ION, 22
4ZINC (II) ION, 32

Entities:

Entity 1, Zinc finger protein 295 107 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYALASERPRO
2   VALGLUASNLYSGLUVALTYRGLNCYSARG
3   LEUCYSASNALALYSLEUSERSERLEULEU
4   GLUGLNGLYSERHISGLUARGLEUCYSARG
5   ASNALAALAVALCYSPROTYRCYSSERLEU
6   ARGPHEPHESERPROGLULEULYSGLNGLU
7   HISGLUSERLYSCYSGLUTYRLYSLYSLEU
8   THRCYSLEUGLUCYSMETARGTHRPHELYS
9   SERSERPHESERILETRPARGHISGLNVAL
10   GLUVALHISASNGLNASNASNMETALAPRO
11   THRSERGLYPROSERSERGLY

Entity 2, ZINC (II) ION, 1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: zf-C2H2 domain, [U-13C; U-15N], 1.03 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; ZnCl2 0.01 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D_13C-separated_NOESYsample_1not availablecondition_1
3D_15N-separated_NOESYsample_1not availablecondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.901, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAA86541 BAA95529 BAD74063 BAD74064 BAE02111
GB AAH63290 AIC56336 EAX09579 EAX09580 EAX09581
REF NP_001091872 NP_001091873 NP_065778 XP_001106387 XP_001106513
SP Q9ULJ3
AlphaFold Q9ULJ3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks