BMRB Entry 10034

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PDB ID: 1wk0
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10034
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of Fibronectin type III domain derived from human KIAA0970 protein

Deposition date:

2006-11-01

Original release date:

2008-08-13

Authors:

Kobayashi, N.; Koshiba, S.; Inoue, M.; Hayashi, F.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Kobayashi, N.; Koshiba, S.; Inoue, M.; Hayashi, F.; Kigawa, T.; Yokoyama, S.. "Solution structure of Fibronectin type III domain derived from human KIAA0970 protein" .

Assembly members:

Assembly members:
Fibronectin type3 domain, polymer, 137 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P030723-24

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Fibronectin type3 domain: GSSGSSGDEETKAFEALLSN IVKPVASDIQARTVVLTWSP PSSLINGETDESSVPELYGY EVLISSTGKDGKYKSVYVGE ETNITLNDLKPAMDYHAKVQ AEYNSIKGTPSEAEIFTTLS CEPDIPNPPRISGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	577
15N chemical shifts	134
1H chemical shifts	907

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	KIAA0970 protein	1

Entities:

Entity 1, KIAA0970 protein 137 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ASP

GLU

2

THR

LYS

ALA

PHE

GLU

ALA

LEU

SER

ASN

3

ILE

VAL

LYS

PRO

VAL

ALA

SER

ASP

ILE

GLN

4

ALA

ARG

THR

VAL

LEU

THR

TRP

SER

PRO

5

PRO

SER

LEU

ILE

ASN

GLY

GLU

THR

ASP

6

GLU

SER

VAL

PRO

GLU

LEU

TYR

GLY

TYR

7

GLU

VAL

LEU

ILE

SER

THR

GLY

LYS

ASP

8

GLY

LYS

TYR

LYS

SER

VAL

TYR

VAL

GLY

GLU

9

GLU

THR

ASN

ILE

THR

LEU

ASN

ASP

LEU

LYS

10

PRO

ALA

MET

ASP

TYR

HIS

ALA

LYS

VAL

GLN

11

ALA

GLU

TYR

ASN

SER

ILE

LYS

GLY

THR

PRO

12

SER

GLU

ALA

GLU

ILE

PHE

THR

LEU

SER

13

CYS

GLU

PRO

ASP

ILE

PRO

ASN

PRO

ARG

14

ILE

SER

GLY

PRO

SER

GLY

Samples:

sample_1: fibronectin type III domain, [U-13C; U-15N], 1.11 mM; Phosphate 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	not available	condition_1
3D 15N-separated NOESY	sample_1	not available	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.901, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Varian UNITY 900 MHz

PDB	1WK0
DBJ	BAA76814 BAG11267 BAG63723
EMBL	CAG44602 CAG44603 CAH90829 CAI45989
GB	AAI32813 AAI36618 AAI44302 EAX08803 EAX08804
REF	NP_001073141 NP_001125472 NP_001265367 NP_055738 XP_001152472
SP	Q5RBN1 Q9Y2H6
AlphaFold	Q5RBN1 Q9Y2H6