BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 10031

Title: Solution structure of the 2nd mbt domain from human KIAA1617 protein

Deposition date: 2006-11-01 Original release date: 2008-08-13

Authors: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.

Citation: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the 2nd mbt domain from human KIAA1617 protein"  .

Assembly members:
mbt domain, polymer, 127 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis   Vector: P040119-21

Entity Sequences (FASTA):
mbt domain: GSSGSSGPIDLITVGSLIEL QDSQNPFQYWIVSVIENVGG RLRLRYVGLEDTESYDQWLF YLDYRLRPVGWCQENKYRMD PPSEIYPLKMASEWKCTLEK SLIDAAKFPLPMEVFKDHAD LSGPSSG

Data sets:
Data typeCount
13C chemical shifts558
15N chemical shifts125
1H chemical shifts878

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1KIAA1617 protein1

Entities:

Entity 1, KIAA1617 protein 127 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYPROILEASP
2   LEUILETHRVALGLYSERLEUILEGLULEU
3   GLNASPSERGLNASNPROPHEGLNTYRTRP
4   ILEVALSERVALILEGLUASNVALGLYGLY
5   ARGLEUARGLEUARGTYRVALGLYLEUGLU
6   ASPTHRGLUSERTYRASPGLNTRPLEUPHE
7   TYRLEUASPTYRARGLEUARGPROVALGLY
8   TRPCYSGLNGLUASNLYSTYRARGMETASP
9   PROPROSERGLUILETYRPROLEULYSMET
10   ALASERGLUTRPLYSCYSTHRLEUGLULYS
11   SERLEUILEASPALAALALYSPHEPROLEU
12   PROMETGLUVALPHELYSASPHISALAASP
13   LEUSERGLYPROSERSERGLY

Samples:

sample_1: mbt domain, [U-13C; U-15N], 1.14 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1not availablecondition_1
3D 15N-separated NOESYsample_1not availablecondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.901, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
REF XP_006717553 XP_008573689 XP_011760994 XP_011760995 XP_011760996

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts