BMRB Entry 10029
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10029
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the fifth PDZ domain of human membrane associated
guanylate kinase inverted-2 (KIAA0705 protein)" .
Assembly members:
PDZ domain, polymer, 103 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Vector: P021030-33
Entity Sequences (FASTA):
PDZ domain: GSSGSSGQDFDYFTVDMEKG
AKGFGFSIRGGREYKMDLYV
LRLAEDGPAIRNGRMRVGDQ
IIEINGESTRDMTHARAIEL
IKSGGRRVRLLLKRGTGSGP
SSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 417 |
| 15N chemical shifts | 102 |
| 1H chemical shifts | 659 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | membrane associated guanylate kinase inverted-2 | 1 |
Entities:
Entity 1, membrane associated guanylate kinase inverted-2 103 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | GLN | ASP | PHE | ||||
| 2 | ASP | TYR | PHE | THR | VAL | ASP | MET | GLU | LYS | GLY | ||||
| 3 | ALA | LYS | GLY | PHE | GLY | PHE | SER | ILE | ARG | GLY | ||||
| 4 | GLY | ARG | GLU | TYR | LYS | MET | ASP | LEU | TYR | VAL | ||||
| 5 | LEU | ARG | LEU | ALA | GLU | ASP | GLY | PRO | ALA | ILE | ||||
| 6 | ARG | ASN | GLY | ARG | MET | ARG | VAL | GLY | ASP | GLN | ||||
| 7 | ILE | ILE | GLU | ILE | ASN | GLY | GLU | SER | THR | ARG | ||||
| 8 | ASP | MET | THR | HIS | ALA | ARG | ALA | ILE | GLU | LEU | ||||
| 9 | ILE | LYS | SER | GLY | GLY | ARG | ARG | VAL | ARG | LEU | ||||
| 10 | LEU | LEU | LYS | ARG | GLY | THR | GLY | SER | GLY | PRO | ||||
| 11 | SER | SER | GLY |
Samples:
sample_1: PDZ domain, [U-13C; U-15N], 1.3 mM; phosphate 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | not available | condition_1 |
| 3D 15N-separated NOESY | sample_1 | not available | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B. A. - data analysis
Kujira v0.8994, Kobayashi, N. - data analysis
CYANA v2.0.17, Guentert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks