BMRB Entry 10028

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PDB ID: 2dwv
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10028
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the second WW domain from mouse salvador homolog 1 protein (mm45).

Deposition date:

2006-10-20

Original release date:

2007-10-19

Authors:

Ohnishi, Satoshi; Kigawa, Takanori; Koshiba, Seizo; Tomizawa, Tadashi; Sato, Manami; Tochio, Naoya; Inoue, Makoto; Harada, Takushi; Watanabe, Satoru; Yokoyama, Shigeyuki

Citation:

Citation: Ohnishi, S.; Guntert, P.; Koshiba, S.; Tomizawa, T.; Akasaka, R.; Tochio, N.; Sato, M.; Inoue, M.; Harada, T.; Watanabe, S.; Tanaka, A.; Shirouzu, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of an atypical WW domain in a novel beta-clam-like dimeric form" Febs Lett. 581, 462-468 (2007).
PubMed: 17239860

Assembly members:

Assembly members:
WW domain, polymer, 49 residues, Formula weight is not available

Natural source:

Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P051121-01

Entity Sequences (FASTA):

Entity Sequences (FASTA):
WW domain: GSSGSSGPLEREGLPPGWER VESSEFGTYYVDHTNKRAQY RHPSGPSSG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shift_1

Data type	Count
13C chemical shifts	184
15N chemical shifts	39
1H chemical shifts	269

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	WW domain 1	1
2	WW domain 2	1

Entities:

Entity 1, WW domain 1 49 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

PRO

LEU

GLU

2

ARG

GLU

GLY

LEU

PRO

GLY

TRP

GLU

ARG

3

VAL

GLU

SER

GLU

PHE

GLY

THR

TYR

4

VAL

ASP

HIS

THR

ASN

LYS

ARG

ALA

GLN

TYR

5

ARG

HIS

PRO

SER

GLY

PRO

SER

GLY

Samples:

sample_1: WW domain, [U-13C; U-15N], 1 mM; d-Tris HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; D2O 10%; H2O 90%

condition_1: ionic strength: 0.12 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.935, Kobayashi, N. - data analysis

CYANA v2.2, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz