BMRB Entry 10026

Title:
Solution structure of kinase associated domain 1 of mouse MAP/microtubule affinity-regulating kinase 3
Deposition date:
2006-09-25
Original release date:
2008-06-24
Authors:
Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Tochio, N.; Koshiba, S.; Kobayashi, N.; Inoue, M.; Yabuki, T.; Aoki, M.; Seki, E.; Matsuda, T.; Tomo, Y.; Motoda, Y.; Kobayashi, A.; Tanaka, A.; Hayashizaki, Y.; Terada, T.; Shirouzu, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the kinase-associated domain 1 of mouse microtubule-associated protein/microtubule affinity-regulating kinase 3"  Protein Sci. 15, 2534-2543 (2006).
PubMed: 17075132

Assembly members:

Assembly members:
Kinase associated domain 1 (KA1), polymer, 126 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P020417-25

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts559
15N chemical shifts141
1H chemical shifts864

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MAP/microtubule affinity-regulating kinase 31

Entities:

Entity 1, MAP/microtubule affinity-regulating kinase 3 126 residues - Formula weight is not available

1   METLYSASPHISLEUILEHISASNVALHIS
2   LYSGLUGLUHISALAHISALAHISASNLYS
3   ASPTYRASPILEPROTHRTHRGLUASNLEU
4   TYRPHEGLNGLYSERSERGLYSERSERGLY
5   ASPMETMETARGGLUILEARGLYSVALLEU
6   GLYALAASNASNCYSASPTYRGLUGLNARG
7   GLUARGPHELEULEUPHECYSVALHISGLY
8   ASPGLYHISALAGLUASNLEUVALGLNTRP
9   GLUMETGLUVALCYSLYSLEUPROARGLEU
10   SERLEUASNGLYVALARGPHELYSARGILE
11   SERGLYTHRSERILEALAPHELYSASNILE
12   ALASERLYSILEALAASNGLULEULYSLEU
13   SERGLYPROSERSERGLY

Samples:

sample_1: KA1 domain, [U-13C; U-15N], 2.0 mM; phosphate 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; D2O 10%; H2O 90%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D_13C-separated_NOESYsample_1not availablecondition_1
3D_15N-separated_NOESYsample_1not availablecondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425 - processing

NMRView v5.0.4 - data analysis

Kujira v0.853 - data analysis

CYANA v2.0.17 - structure solution & refinement

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
REF XP_002696830 XP_005200933 XP_005200934 XP_005200936 XP_005200937
TPG DAA17379

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks