BMRB Entry 10019

Name: GSPT1/eRF3a(PAM2-1)-PABC complex
Published: 2010-05-27

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PDB ID: 2rqg
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: LACS
BMRB Entry DOI: doi:10.13018/BMR10019
MolProbity Validation Chart

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

GSPT1/eRF3a(PAM2-1)-PABC complex

Deposition date:

2006-03-27

Original release date:

2010-05-27

Authors:

Osawa, Masanori; Shimada, Ichio

Citation:

Citation: Osawa, Masanori; Shimada, Ichio. "1H and 15N Assigned Chemical Shifts for GSPT1/eRF3a(PAM2-1) and 1H, 13C and 15N Assigned Chemical Shifts for PABC" J. Biol. Chem. ., .-..

Assembly members:

Assembly members:
PAM2-1, polymer, 19 residues, Formula weight is not available
PABC domain, polymer, 83 residues, Formula weight is not available

Natural source:

Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculas

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PAM2-1: AFSRQLNVNAKPFVPNVHA
PABC domain: GQEPLTASMLASAPPQEQKQ MLGERLFPLIQAMHPTLAGK ITGMLLEIDNSELLHMLESP ESLRSKVDEAVAVLQAHQAK EAA

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	257
15N chemical shifts	77
1H chemical shifts	674

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PAM2-1	1
2	PABC	2

Entities:

Entity 1, PAM2-1 19 residues - Formula weight is not available

1

ALA

PHE

SER

ARG

GLN

LEU

ASN

VAL

ASN

ALA

2

LYS

PRO

PHE

VAL

PRO

ASN

VAL

HIS

ALA

Entity 2, PABC 83 residues - Formula weight is not available

1

GLY

GLN

GLU

PRO

LEU

THR

ALA

SER

MET

LEU

2

ALA

SER

ALA

PRO

GLN

GLU

GLN

LYS

GLN

3

MET

LEU

GLY

GLU

ARG

LEU

PHE

PRO

LEU

ILE

4

GLN

ALA

MET

HIS

PRO

THR

LEU

ALA

GLY

LYS

5

ILE

THR

GLY

MET

LEU

GLU

ILE

ASP

ASN

6

SER

GLU

LEU

HIS

MET

LEU

GLU

SER

PRO

7

GLU

SER

LEU

ARG

SER

LYS

VAL

ASP

GLU

ALA

8

VAL

ALA

VAL

LEU

GLN

ALA

HIS

GLN

ALA

LYS

9

GLU

ALA

Samples:

sample_1: PAM2-1, unlabeled, 1 mM; PABC domain, [U-95% 13C, U-95% 15N], 1 mM; NaCl 150 mM

conditions_1: ionic strength: 0.15 M; pH: 6.5; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
15N-1H HSQC	sample_1	not available	conditions_1
13C-1H HSQC	sample_1	not available	conditions_1
CBCA(CO)NH	sample_1	not available	conditions_1
HNCACB	sample_1	not available	conditions_1
C(CO)NH	sample_1	not available	conditions_1
H(CCO)NH	sample_1	not available	conditions_1
HBHA(CBCACO)NH	sample_1	not available	conditions_1
HCCH-TOCSY	sample_1	not available	conditions_1
15N-edited TOCSY HSQC	sample_1	not available	conditions_1
15N-edited NOESY HSQC	sample_1	not available	conditions_1
13C,15N-filterd NOESY	sample_1	not available	conditions_1
13C,15N-filterd TOCSY	sample_1	not available	conditions_1

Software:

No software information available

NMR spectrometers:

Bruker Avance 600 MHz

PDB	2RQG 3KUI
DBJ	BAA92160 BAE22025 BAJ20641 BAT46595
EMBL	CAG31442 CAH93403
GB	AAH09503 AAH31640 AAH77825 AAH79092 AAH90081
REF	NP_001003978 NP_001015805 NP_001086960 NP_001091540 NP_001123478
SP	Q5R4B3 Q8R050
TPG	DAA15601
AlphaFold	Q5R4B3 Q8R050