BMRB Entry 10013

Name: Solution Structure Of The Ring Finger Domain Of The Human Kiaa1045 Protein
Published: 2006-12-06

Click here to enlarge.

PDB ID: 1wil
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10013
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Solution Structure Of The Ring Finger Domain Of The Human Kiaa1045 Protein

Deposition date:

2005-11-20

Original release date:

2006-12-06

Authors:

Miyamoto, K.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Miyamoto, K.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution Structure of the RING Finger Domain of the Human KIAA1045 Protein" not known ., .-..

Assembly members:

Assembly members:
RING Finger Domain, polymer, 89 residues, 9688.757 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P030609-29

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RING Finger Domain: GSSGSSGPREPVVNDEMCDV CEVWTAESLFPCRVCTRVFH DGCLRRMGYIQGDSAAEVTE MAHTETGWSCHYCDNINLLL TEESGPSSG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shift_1

Data type	Count
13C chemical shifts	340
15N chemical shifts	76
1H chemical shifts	519

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RING Finger Domain	1
2	ZINC (II) ION, 1	2
3	ZINC (II) ION, 2	2

Entities:

Entity 1, RING Finger Domain 89 residues - 9688.757 Da.

1

GLY

SER

GLY

SER

GLY

PRO

ARG

GLU

2

PRO

VAL

ASN

ASP

GLU

MET

CYS

ASP

VAL

3

CYS

GLU

VAL

TRP

THR

ALA

GLU

SER

LEU

PHE

4

PRO

CYS

ARG

VAL

CYS

THR

ARG

VAL

PHE

HIS

5

ASP

GLY

CYS

LEU

ARG

MET

GLY

TYR

ILE

6

GLN

GLY

ASP

SER

ALA

GLU

VAL

THR

GLU

7

MET

ALA

HIS

THR

GLU

THR

GLY

TRP

SER

CYS

8

HIS

TYR

CYS

ASP

ASN

ILE

ASN

LEU

9

THR

GLU

SER

GLY

PRO

SER

GLY

Entity 2, ZINC (II) ION, 1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: RING Finger Domain, [U-13C; U-15N], 0.4 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.1 mM; D2O 10%; H2O 90%

condition_1: ionic strength: 0.12 M; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D_13C-separated_NOESY	sample_1	not available	condition_1
3D_15N-separated_NOESY	sample_1	not available	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425 - processing

NMRView v5.0.4 - data analysis

Kujira v0.8996 - data analysis

CYANA v2.0.17 - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	1WIL
DBJ	BAA82997 BAE90222 BAE90573 BAG09947 BAH11739
EMBL	CAH93249 CAI29683
GB	EAW58408 EHH24010 EHH57286 ELR52775
REF	NP_001127105 NP_001128900 NP_001291262 NP_056112 XP_002689719
SP	Q5R4R7 Q9UPV7
TPG	DAA26823
AlphaFold	Q5R4R7 Q9UPV7