BMRB Entry 10010

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10010

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Title:
bovine beta-lactoglobulin A34C mutant
Deposition date:
2005-10-31
Original release date:
2007-06-13
Authors:
Sakurai, Kazumasa; Goto, Yuji
Citation:

Citation: Sakurai, Kazumasa; Goto, Yuji. "Dynamics and Mechanism of the Tanford Transition of Bovine beta-Lactoglobulin Studied using Heteronuclear NMR Spectroscopy"  J. Mol. Biol. 356, 483-496 (2006).
PubMed: 16368109

Assembly members:

Assembly members:
BLG_A34C, polymer, 165 residues, 18400 Da.

Natural source:

Natural source:   Common Name: Cow   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pPIC9

Entity Sequences (FASTA):

Entity Sequences (FASTA):
BLG_A34C: EAEAYVTQTMKGLDIQKVAG TWYSLAMAASDISLLDCQSA PLRVYVEELKPTPEGDLEIL LQKWENDECAQKKIIAEKTK IPAVFKIDALNENKVLVLDT DYKKYLLFCMENSAEPEQSL VCQCLVRTPEVDDEALEKFD KALKALPMHIRLSFNPTQLE EQCHI

Data sets:
Data typeCount
13C chemical shifts463
15N chemical shifts147
1H chemical shifts147

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1BLG A34C, 11
2BLG A34C, 21

Entities:

Entity 1, BLG A34C, 1 165 residues - 18400 Da.

The first three residues are signal sequence necessary for the expression. The native sequence numbering starts at the forth residue. -3 EAE -1 1 AYVTQ TMKGL DIQKV AGTWY 20 21 SLAMA ASDIS LLDAQ SAPLR 40 41 VYVEE LKPTP EGDLE ILLQK 60 61 WENDE CAQKK IIAEK TKIPA 80 81 VFKID ALNEN KVLVL DTDYK 100 101 KYLLF CMENS AEPEQ SLVCQ 120 121 CLVRT PEVDD EALEK FDKAL 140 141 KALPM HIRLS FNPTQ LEEQC 160 161 HI 162

1   GLUALAGLUALATYRVALTHRGLNTHRMET
2   LYSGLYLEUASPILEGLNLYSVALALAGLY
3   THRTRPTYRSERLEUALAMETALAALASER
4   ASPILESERLEULEUASPCYSGLNSERALA
5   PROLEUARGVALTYRVALGLUGLULEULYS
6   PROTHRPROGLUGLYASPLEUGLUILELEU
7   LEUGLNLYSTRPGLUASNASPGLUCYSALA
8   GLNLYSLYSILEILEALAGLULYSTHRLYS
9   ILEPROALAVALPHELYSILEASPALALEU
10   ASNGLUASNLYSVALLEUVALLEUASPTHR
11   ASPTYRLYSLYSTYRLEULEUPHECYSMET
12   GLUASNSERALAGLUPROGLUGLNSERLEU
13   VALCYSGLNCYSLEUVALARGTHRPROGLU
14   VALASPASPGLUALALEUGLULYSPHEASP
15   LYSALALEULYSALALEUPROMETHISILE
16   ARGLEUSERPHEASNPROTHRGLNLEUGLU
17   GLUGLNCYSHISILE

Samples:

sample_1: beta-lactoglobulin A34C polypeptide, [U-99% 13C; U-99.3% 15N], 2 ± 0.2 mM; Hydrochloric acid7 – 9 mM

conditions_for_BLG_A34C_at_pH_6.5: ionic strength: 0.000 M; pH: 6.5; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
1H15N HSQCsample_1not availableconditions_for_BLG_A34C_at_pH_6.5
CBCA(CO)NHsample_1not availableconditions_for_BLG_A34C_at_pH_6.5
HNCACBsample_1not availableconditions_for_BLG_A34C_at_pH_6.5
HNCOsample_1not availableconditions_for_BLG_A34C_at_pH_6.5
HNCACOsample_1not availableconditions_for_BLG_A34C_at_pH_6.5

Software:

pipp.com -

NMR spectrometers:

  • Bruker DRX 800 MHz

Related Database Links:

PDB
EMBL CAA06532 CAA32835 CAA88303 CAY39357
GB AAA30411 AAI08214 ABF48380 ACG59280 AFB74990
PRF 0601265A
REF NP_001277893 NP_776354 XP_005888577 XP_006062245
SP P02754 P02755
TPG DAA24277
AlphaFold P02754 P02755

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks