BMRB Entry 10009

Name: Solution structure of SH3 domain of mouse Kalirin-9a protein
Published: 2006-11-06

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PDB ID: 1wfw
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10009
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of SH3 domain of mouse Kalirin-9a protein

Deposition date:

2005-10-25

Original release date:

2006-11-06

Authors:

Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of SH3 domain of mouse Kalirin-9a protein" not known ., .-..

Assembly members:

Assembly members:
SH3 domain, polymer, 74 residues, 7641.529 Da.

Natural source:

Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P030120-92

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SH3 domain: GSSGSSGSTMTVIKDYYALK ENEICVSQGEVVQVLAVNQQ NMCLVYQPASDHSPAAEGWV PGSILAPFSGPSSG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shift_1

Data type	Count
13C chemical shifts	284
15N chemical shifts	66
1H chemical shifts	449

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SH3 domain	1

Entities:

Entity 1, SH3 domain 74 residues - 7641.529 Da.

1

GLY

SER

GLY

SER

GLY

SER

THR

MET

2

THR

VAL

ILE

LYS

ASP

TYR

ALA

LEU

LYS

3

GLU

ASN

GLU

ILE

CYS

VAL

SER

GLN

GLY

GLU

4

VAL

GLN

VAL

LEU

ALA

VAL

ASN

GLN

5

ASN

MET

CYS

LEU

VAL

TYR

GLN

PRO

ALA

SER

6

ASP

HIS

SER

PRO

ALA

GLU

GLY

TRP

VAL

7

PRO

GLY

SER

ILE

LEU

ALA

PRO

PHE

SER

GLY

8

PRO

SER

GLY

Samples:

sample_1: SH3 domain, [U-13C; U-15N], 1 mM; d-Tris HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; D2O 10%; H2O 90%

condition_1: ionic strength: 0.12 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	not available	condition_1
3D 15N-separated NOESY	sample_1	not available	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20031121 - processing

NMRView v5.0.4 - data analysis

Kujira v0.8994 - data analysis

CYANA v2.0.17 - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz