BMRB Entry 10005

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10005

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Title:
1H, 13C, and 15N Chemical Shift Assignments for ChitinaseC chitin binding domain
Deposition date:
2005-10-12
Original release date:
2007-06-13
Authors:
Akagi, Ken-ichi; Ikegami, Takahisa
Citation:

Citation: Akagi, Ken-ichi; Watanabe, Jun; Hara, Masashi; Kezuka, Yuichiro; Chikaishi, Eriko; Yamaguchi, Tohru; Akutsu, Hideo; Nonaka, Takamasa; Watanabe, Takeshi; Takahisa, Ikegami. "Identification of the Substrate Interaction Region of the Chitin-Binding Domain of Streptomyces griseus Chitinase C"  J. Biochem. 139, 483-493 (2006).
PubMed: 16567413

Assembly members:

Assembly members:
ChitinaseC chitin binding domain, polymer, 54 residues, 5741 Da.

Natural source:

Natural source:   Common Name: Streptomyces griseinus   Taxonomy ID: 66893   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptomyces griseinus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ChitinaseC chitin binding domain: MATCATAWSSSSVYTNGGTV SYNGRNYTAKWWTQNERPGT SDVWADKGACGTGS

Data sets:
Data typeCount
13C chemical shifts188
15N chemical shifts61
1H chemical shifts301

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ChBD1

Entities:

Entity 1, ChBD 54 residues - 5741 Da.

1   METALATHRCYSALATHRALATRPSERSER
2   SERSERVALTYRTHRASNGLYGLYTHRVAL
3   SERTYRASNGLYARGASNTYRTHRALALYS
4   TRPTRPTHRGLNASNGLUARGPROGLYTHR
5   SERASPVALTRPALAASPLYSGLYALACYS
6   GLYTHRGLYSER

Samples:

sample_1: ChitinaseC chitin binding domain, [U-13C; U-15N], 1.0 mM

condition_1: pH: 6.0; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCsample_1not availablecondition_1
1H-13C HSQCsample_1not availablecondition_1
1H-1H TOCSYsample_1not availablecondition_1
1H-1H NOESYsample_1not availablecondition_1
NOESY-15N-HSQCsample_1not availablecondition_1
TOCSY-15N-HSQCsample_1not availablecondition_1
HNCOsample_1not availablecondition_1
HN(CA)COsample_1not availablecondition_1
CBCA(CO)NHsample_1not availablecondition_1
CBCANHsample_1not availablecondition_1
HBHA(CBCACO)NHsample_1not availablecondition_1
HCCH-TOCSYsample_1not availablecondition_1
C(CO)NHsample_1not availablecondition_1
H(CCO)NHsample_1not availablecondition_1
(HB)CB(CGCD)HDsample_1not availablecondition_1
(HB)CB(CGCDCE)HEsample_1not availablecondition_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX500 500 MHz
  • Bruker DRX600 600 MHz

Related Database Links:

PDB
DBJ BAA23739 BAG20229
GB ADQ43777 EGE43002 KND35043 KPL31411 KQX37120
REF WP_003967541 WP_026291187 WP_050358467

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks