BMRB Entry 10004

Title:
1H, 13C and 15N resonance assignments of the 2'-5' RNA ligase-like protein from Pyrococcus furiosus
Deposition date:
2005-10-05
Original release date:
2006-04-27
Authors:
Okada, Kiyoshi; Matsuda, Takashi; Sakamoto, Taiichi; Muto, Yutaka; Yokoyama, Shigeyuki; Kanai, Akio; Kawai, Gota
Citation:

Citation: Okada, Kiyoshi; Matsuda, Takashi; Sakamoto, Taiichi; Muto, Yutaka; Yokoyama, Shigeyuki; Kanai, Akio; Kawai, Gota. "1H, 13C and 15N resonance assignments of the 2'-5' RNA ligase-like protein from Pyrococcus furiosus"  J. Biomol. NMR 36, 16-16 (2006).
PubMed: 16456701

Assembly members:

Assembly members:
2'-5' RNA ligase-like protein, polymer, 190 residues, 21793 Da.

Natural source:

Natural source:   Common Name: Pyrococcus furiosus   Taxonomy ID: 186497   Superkingdom: Archaea   Kingdom: not available   Genus/species: Pyrococcus Pyrococcus furiosus

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
  • assigned_chemical_shifts
Data typeCount
13C chemical shifts894
15N chemical shifts191
1H chemical shifts1434

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
12'-5' RNA ligase-like protein1

Entities:

Entity 1, 2'-5' RNA ligase-like protein 190 residues - 21793 Da.

1   METARGALAPHEILEALAILEASPVALSER
2   GLUSERVALARGASPALALEUVALARGALA
3   GLNASPTYRILEGLYSERLYSGLUALALYS
4   ILELYSPHEVALGLUARGGLUASNPHEHIS
5   ILETHRLEULYSPHELEUGLYGLUILETHR
6   GLUGLUGLNALAGLUGLUILELYSLYSILE
7   LEUGLULYSILEALALYSLYSTYRLYSLYS
8   HISGLUVALASNVALARGGLYILEGLYVAL
9   PHEPROASNPROASNTYRVALARGVALILE
10   TRPALAGLYVALGLUASNASPGLUILEILE
11   LYSLYSILEALALYSGLUILEASPASPGLU
12   LEUALALYSLEUGLYPHELYSLYSGLUGLY
13   ASNPHEVALALAHISILETHRLEUGLYARG
14   VALLYSPHEVALLYSASPLYSLEUGLYLEU
15   ALAMETLYSLEULYSGLULEUALAASNGLU
16   ASPPHEGLYSERPHEILEVALGLUALAILE
17   GLULEULYSLYSSERTHRLEUTHRPROLYS
18   GLYPROILETYRGLUTHRLEUALAARGPHE
19   GLULEUSERGLUHISHISHISHISHISHIS

Samples:

pf0027_1: 2'-5' RNA ligase-like protein, [U-13C; U-15N], 1.1 ± 0.1 mM; hydrochloric acid 1.0 ± 0.1 mM; sodium chloride 200 ± 0.1 mM

condition_1: pH: 4.7; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCpf0027_1not availablecondition_1
1H-13C HSQCpf0027_1not availablecondition_1
HNCOpf0027_1not availablecondition_1
HNCACOpf0027_1not availablecondition_1
HNCOCApf0027_1not availablecondition_1
HNCApf0027_1not availablecondition_1
CBCACONHpf0027_1not availablecondition_1
HNCACBpf0027_1not availablecondition_1
CC(CO)NHpf0027_1not availablecondition_1
HCCH-TOCSYpf0027_1not availablecondition_1
H(CCCO)NHpf0027_1not availablecondition_1
HCCH-COSYpf0027_1not availablecondition_1
HNCBpf0027_1not availablecondition_1

Software:

No software information available

NMR spectrometers:

  • Bruker AVANCE 700 MHz

Related Database Links:

PDB
GB AAL80151 AFN04547
REF WP_011011139
SP Q8U4Q3
AlphaFold Q8U4Q3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks