BMRB Entry 7321

Title:
Complete 1H 13C 15N chemical shift assignments for LDLa module from RXFP1
Deposition date:
2006-10-22
Original release date:
2006-11-06
Authors:
Hopkins, Emma; Gooley, Paul
Citation:

Citation: Hopkins, Emma; Layfield, Sharon; Ferraro, Tania; Bathgate, Ross; Gooley, Paul. "The NMR solution structure of the relaxin (RXFP1) receptor lipoprotein receptor class A module and identification of key residues in the N-terminal region of the module that mediate receptor activation."  J. Biol. Chem. 282, 4172-4184 (2007).
PubMed: 17148455

Assembly members:

Assembly members:
LDLa, polymer, 43 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
LDLa: GSQDVKCSLGYFPCGNITKC LPQLLHCNGVDDCGNQADED NCG

Data sets:
Data typeCount
13C chemical shifts100
15N chemical shifts43
1H chemical shifts242

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1LDLa module of RXFP11
2CALCIUM (II) ION2

Entities:

Entity 1, LDLa module of RXFP1 43 residues - Formula weight is not available

1   GLYSERGLNASPVALLYSCYSSERLEUGLY
2   TYRPHEPROCYSGLYASNILETHRLYSCYS
3   LEUPROGLNLEULEUHISCYSASNGLYVAL
4   ASPASPCYSGLYASNGLNALAASPGLUASP
5   ASNCYSGLY

Entity 2, CALCIUM (II) ION - Ca - 40.078 Da.

1   CA

Samples:

sample_1: LDLa, [U-13C; U-15N], 1.5 ± 0.1 mM; soium acetate 50 mM; calcium chloride 10 mM; H2O 90%; D2O 10%

sample_2: LDLa, [U-15N], 1 ± 0.1 mM; soium acetate 50 mM; calcium chloride 10 mM; H2O 90%; D2O 10%

conditions_1: pH: 5.5; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H15N HSQCsample_2not availableconditions_1
CBCA(CO)NHsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
C(CO)NHsample_1not availableconditions_1
HC(CO)NHsample_1not availableconditions_1
HCCH-TOCSYsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
HNHAsample_2not availableconditions_1
HNHBsample_2not availableconditions_1

Software:

NMRPipe v2.3, Frank Delaglio - processing

SPARKY v3.11, Goddard and Kneller - analysis

NMR spectrometers:

  • Varian Inova 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks