BMRB Entry 7306

Title:
R21A Spc-SH3 bound
Deposition date:
2006-09-28
Original release date:
2007-05-04
Authors:
van Nuland, Nico; Casares, Salvador; AB, Eiso; Eshuis, Henk; Lopez-Mayorga, Obdulio; Conejero-Lara, Francisco
Citation:

Citation: Casares, Salvador; AB, Eiso; Eshuis, Henk; Lopez-Mayorga, Obdulio; van Nuland, Nico; Conejero-Lara, Francisco. "The high-resolution NMR structure of the R21A Spc-SH3:P41 complex: Understanding the determinants of binding affinity by comparison with Abl-SH3"  BMC Struct. Biol. 7, .-. (2007).
PubMed: 17407569

Assembly members:

Assembly members:
R21A Spc-SH3 bound, polymer, 62 residues, Formula weight is not available
p41-SH3, polymer, 11 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
R21A Spc-SH3 bound: MDETGKELVLALYDYQEKSP AEVTMKKGDILTLLNSTNKD WWKVEVNDRQGFVPAAYVKK LD
p41-SH3: XAPSYSPPPPP

Data sets:
Data typeCount
1H chemical shifts471

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1R21A Spc-SH3 monomer1
2polyproline_ligand2

Entities:

Entity 1, R21A Spc-SH3 monomer 62 residues - Formula weight is not available

1   METASPGLUTHRGLYLYSGLULEUVALLEU
2   ALALEUTYRASPTYRGLNGLULYSSERPRO
3   ALAGLUVALTHRMETLYSLYSGLYASPILE
4   LEUTHRLEULEUASNSERTHRASNLYSASP
5   TRPTRPLYSVALGLUVALASNASPARGGLN
6   GLYPHEVALPROALAALATYRVALLYSLYS
7   LEUASP

Entity 2, polyproline_ligand 11 residues - Formula weight is not available

1   ACEALAPROSERTYRSERPROPROPROPRO
2   PRO

Samples:

sample_2: R21A Spc-SH3 bound 2 mM; P41 5.2; H2O 90%; D2O 10%; d5-glycine 20 mM

conditions_1: pH: 3.5; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D TOCSYsample_2not availableconditions_1
2D NOESYsample_2not availableconditions_1

Software:

NMRView v5 -

NMR spectrometers:

  • Bruker DRX 750MHz MHz