BMRB Entry 7287

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7287
MolProbity Validation Chart

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Title:
Backbone and side chain 1H, 13C and 15N Chemical Shift Assignments for human BRD7 bromodomain
Deposition date:
2006-09-02
Original release date:
2007-01-10
Authors:
Sun, Hongbin; Liu, Jiangxin; Zhang, Jiahai; Huang, Hongda; Xu, Chao; Dai, Haiming; Wu, Jihui; Shi, Yunyu
Citation:

Citation: Sun, Hongbin; Liu, Jiangxin; Zhang, Jiahai; Shen, Weiqun; Huang, Hongda; Xu, Chao; Dai, Haiming; Wu, Jihui; Shi, Yunyu. "Solution structure of BRD7 bromodomain and its interaction with acetylated peptides from histone H3 and H4."  Biochem. Biophys. Res. Commun. 358, 435-441 (2007).
PubMed: 17498659

Assembly members:

Assembly members:
bromodomain, polymer, 117 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
bromodomain: MEEVEQTPLQEALNQLMRQL QRKDPSAFFSFPVTDFIAPG YSMIIKHPMDFSTMKEKIKN NDYQSIEELKDNFKLMCTNA MIYNKPETIYYKAAKKLLHS GMKILSQERLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts440
15N chemical shifts116
1H chemical shifts786

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1bromodomain1

Entities:

Entity 1, bromodomain 117 residues - Formula weight is not available

1   METGLUGLUVALGLUGLNTHRPROLEUGLN
2   GLUALALEUASNGLNLEUMETARGGLNLEU
3   GLNARGLYSASPPROSERALAPHEPHESER
4   PHEPROVALTHRASPPHEILEALAPROGLY
5   TYRSERMETILEILELYSHISPROMETASP
6   PHESERTHRMETLYSGLULYSILELYSASN
7   ASNASPTYRGLNSERILEGLUGLULEULYS
8   ASPASNPHELYSLEUMETCYSTHRASNALA
9   METILETYRASNLYSPROGLUTHRILETYR
10   TYRLYSALAALALYSLYSLEULEUHISSER
11   GLYMETLYSILELEUSERGLNGLUARGLEU
12   GLUHISHISHISHISHISHIS

Samples:

sample_1: bromodomain, [U-13C; U-15N], 0.6 mM; phosphate buffer 50 mM; NaCl 50 mM; DTT 1 mM; EDTA 5 mM; H2O 90%; D2O 10%

condition_1: pH: 6.1; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
1H15N HSQCsample_1not availablecondition_1
15N TOCSYsample_1not availablecondition_1
CBCACONHsample_1not availablecondition_1
CBCANHsample_1not availablecondition_1
C(CO)NH-TOCSYsample_1not availablecondition_1
H(CCO)NH-TOCSYsample_1not availablecondition_1
HBHA(CBCACO)NHsample_1not availablecondition_1
HNCOsample_1not availablecondition_1
HN(CA)COsample_1not availablecondition_1
3D15N-separted NOESYsample_1not availablecondition_1
3D3C-separted NOESYsample_1not availablecondition_1
3D HCCH-TOCSYsample_1not availablecondition_1
3D HCCH-COSYsample_1not availablecondition_1

Software:

XWIN-NMR v3.5 -

NMR spectrometers:

  • Bruker DMX 600M MHz

Related Database Links:

PDB
DBJ BAE23823 BAI45636
EMBL CAB72445 CAH92000
GB AAC33302 AAF19526 AAF75126 AAH50728 AAH94706
REF NP_001096730 NP_001167455 NP_036177 NP_037395 XP_001083389
SP O88665 Q5R8B0 Q9NPI1
TPG DAA20095
AlphaFold Q5R8B0 Q9NPI1 O88665

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks