BMRB Entry 7187

Title:
NMR asigment of Ct-ole e 9, C terminal domain from the olive allergen Ole e 9
Deposition date:
2006-06-21
Original release date:
2006-10-02
Authors:
Castrillo, Ines; Trevino, Miguel; Palomares, Oscar; Villalba, Mayte; Rodriguez, Rosalia; Rico, Manuel; Santoro, Jorge; Bruix, Marta
Citation:

Citation: Castrillo, Ines; Trevino, Miguel; Palomares, Oscar; Rico, Manuel; Santoro, Jorge; Bruix, Marta. "NMR assignment of the C-terminal domain of Ole e 9, a major allergen of olive tree pollen"  J. Biomol. NMR 36, 67-67 (2006).
PubMed: 17031524

Assembly members:

Assembly members:
rCtD, polymer, 101 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: olive tree   Taxonomy ID: 4146   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Olea europea

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pPICZ-alpha a

Data sets:
Data typeCount
13C chemical shifts376
15N chemical shifts102
1H chemical shifts618

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ct ole e 91

Entities:

Entity 1, Ct ole e 9 101 residues - Formula weight is not available

1   ALATHRPROTHRPROTHRPROLYSALAALA
2   GLYSERTRPCYSVALPROLYSPROGLYVAL
3   SERASPASPGLNLEUTHRGLYASNILEASN
4   TYRALACYSSERGLNGLYILEASPCYSGLY
5   PROILEGLNPROGLYGLYALACYSPHEGLU
6   PROASNTHRVALLYSALAHISALAALATYR
7   VALMETASNLEUTYRTYRGLNHISALAGLY
8   ARGASNSERTRPASNCYSASPPHESERGLN
9   THRALATHRLEUTHRASNTHRASNPROSER
10   TYRGLYALACYSASNPHEPROSERGLYSER
11   ASN

Samples:

13C15Nolee9-H2O: rCtD, [U-13C; U-15N], 0.7 mM; H20 90%; D20 10%

15Nolee9-H2O: rCtD, [U-15N], 0.7 mM; H20 90%; D20 10%

olee9-H2O: rCtD 0.7 mM; H20 90%; D20 10%

olee9-D2O: rCtD 0.7 mM; D20 100%

conditions_1: pH: 6; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N13C HNCAnot availablenot availableconditions_1
1H15N13C HNCOCAnot availablenot availableconditions_1
1H15N13C CBCANHnot availablenot availableconditions_1
1H15N13C CBCACONHnot availablenot availableconditions_1
1H15N HSQCnot availablenot availableconditions_1
1H13C HSQCnot availablenot availableconditions_1
1H13C HCCH TOCSYnot availablenot availableconditions_1
1H TOCSYnot availablenot availableconditions_1
multiplicity edited 1H13C HSQCnot availablenot availableconditions_1
1H15N NOESY HSQCnot availablenot availableconditions_1
1H13C NOESY HSQCnot availablenot availableconditions_1
1H COSYnot availablenot availableconditions_1
1H NOESYnot availablenot availableconditions_1

Software:

TOPSPIN v1.3, Bruker -

SPARKY v3.111 -

xwinnmr, Bruker -

NMR spectrometers:

  • Bruker AV-800 800 MHz
  • Bruker AV-600 600 MHz

Related Database Links:

PDB
GB AAK58515
SP Q94G86
AlphaFold Q94G86

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks